- Featured Product
- KD/KO Validated
TLR4 Monoclonal antibody
TLR4 Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat, pig
Applications
WB, IP, IF, IHC, CoIP, ELISA
Conjugate
Unconjugated
110
CloneNo.
3G9A4
Cat no : 66350-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | RAW 264.7 cells, pig liver tissue, NIH/3T3 cells, rat liver tissue |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 103 publications below |
| IHC | See 4 publications below |
| IF | See 10 publications below |
| IP | See 1 publications below |
| CoIP | See 2 publications below |
Product Information
66350-1-Ig targets TLR4 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | TLR4 fusion protein Ag13857 |
| Full Name | toll-like receptor 4 |
| Calculated Molecular Weight | 839 aa, 96 kDa |
| Observed Molecular Weight | 100-110 kDa |
| GenBank Accession Number | BC117422 |
| Gene Symbol | TLR4 |
| Gene ID (NCBI) | 7099 |
| RRID | AB_2881730 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TLR4, also named as CD284, belongs to the Toll-like receptor family. TLR4 interacts with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). TLR4 acts via MYD88, TIRAP and TRAF6, leading to NF-kB activation, cytokine secretion and the inflammatory response. Three alternatively spliced transcript variants that encode different protein isoforms have been described.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TLR4 antibody 66350-1-Ig | Download protocol |
| IHC protocol for TLR4 antibody 66350-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation | ||
Adv Sci (Weinh) OR11H1 Missense Variant Confers the Susceptibility to Vogt-Koyanagi-Harada Disease by Mediating Gadd45g Expression | ||
Acta Biomater An injectable co-assembled hydrogel blocks reactive oxygen species and inflammation cycle resisting myocardial ischemia-reperfusion injury. | ||
Cell Rep METTL3-mediated m6A mRNA methylation regulates neutrophil activation through targeting TLR4 signaling | ||
Int J Biol Macromol Angelica sinensis aboveground part polysaccharide and its metabolite 5-MT ameliorate colitis via modulating gut microbiota and TLR4/MyD88/NF-κB pathway | ||
Gut Microbes Microbial hydrogen economy alleviates colitis by reprogramming colonocyte metabolism and reinforcing intestinal barrier. |
