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TRAIL Polyclonal antibody

TRAIL Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 10399-1-AP

Synonyms

Apo 2 ligand, Apo 2L, APO2L, CD253, Protein TRAIL, TL2, TNFSF10, TRAIL

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using TRAIL Polyclonal antibody (10399-1-AP)

    WB analysis of K-562 using 10399-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 10399-1-AP (TRAIL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

10399-1-AP targets TRAIL in WB,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRAIL fusion protein Ag0627
Full Name tumor necrosis factor (ligand) superfamily, member 10
Calculated Molecular Weight 281 aa, 33 kDa
GenBank Accession NumberBC032722
Gene Symbol TNFSF10
Gene ID (NCBI) 8743
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRAIL is a 30 kD protein known as TNF-related apoptosis inducing ligand, CD253, TNFSF10, or Apo-2L. It is expressed as two kinds of forms: cell surface protein (30 kD) and soluble ligand (19-20 kD) produced by megakaryocytes and platelets, monocytes, neutrophils, NK cells and activated T cells. This antigen is also expressed on tumor cells and transformed cell lines. TRAIL has been reported to induce apoptosis in tumor and transformed cell lines by a caspase-dependent process.

Publications

SpeciesApplicationTitle
humanWB

Technol Cancer Res Treat

Nicotine Induces Progressive Properties of Lung Adenocarcinoma A549 Cells by Inhibiting Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) Expression and Plasma Membrane Localization.

Authors - Hui Li
View Article