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TRAK1 Polyclonal antibody

TRAK1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13987-1-AP

Synonyms

KIAA1042, OIP106, TRAK1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRAK1 Polyclonal antibody (13987-1-AP)

    WB analysis using 13987-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13987-1-AP (TRAK1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using TRAK1 Polyclonal antibody (13987-1-AP)

    IHC staining of human stomach using 13987-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13987-1-AP (TRAK1 antibody) at dilution of 1:200 (under 10x lens)..

  • Immunohistochemistry (IHC) staining of human stomach tissue using TRAK1 Polyclonal antibody (13987-1-AP)

    IHC staining of human stomach using 13987-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13987-1-AP (TRAK1 antibody) at dilution of 1:200 (under 40x lens)..

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TRAK1 Polyclonal antibody (13987-1-AP)

    IHC staining of human breast cancer using 13987-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13987-1-AP (TRAK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TRAK1 Polyclonal antibody (13987-1-AP)

    IHC staining of human breast cancer using 13987-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13987-1-AP (TRAK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, MNK-45 cells
Positive IHC detected inhuman stomach tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:800
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

13987-1-AP targets TRAK1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRAK1 fusion protein Ag5055
Full Name trafficking protein, kinesin binding 1
Calculated Molecular Weight 953 aa, 106 kDa
Observed Molecular Weight 64 kDa
GenBank Accession NumberBC015922
Gene Symbol TRAK1
Gene ID (NCBI) 22906
RRIDAB_2877999
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TRAK1 antibody 13987-1-APDownload protocol
IHC protocol for TRAK1 antibody 13987-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Front Neurosci

O-GlcNAcylation is crucial for sympathetic neuron development, maintenance, functionality and contributes to peripheral neuropathy

Authors - Hsueh-Fu Wu
View Article