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KD/KO Validated

TRIM22 Polyclonal antibody

Name: TRIM22 Polyclonal antibody
Brand: Proteintech
SKU: 13744-1-AP

TRIM22 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 13744-1-AP

Synonyms

GPSTAF50, RING finger protein 94, RNF94, Staf 50, STAF50, TRIM22, tripartite motif containing 22

Validation Data Gallery

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AllIHC

IHC staining of human lung cancer using 13744-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13744-1-AP (TRIM22 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 13744-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13744-1-AP (TRIM22 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 13744-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13744-1-AP (TRIM22 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 13744-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13744-1-AP (TRIM22 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 13744-1-AP

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Tested Applications

Positive IHC detected in

human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Application	Dilution
Immunohistochemistry (IHC)	IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 5 publications below
IHC	See 3 publications below
IF	See 1 publications below

Product Information

13744-1-AP targets TRIM22 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TRIM22 fusion protein Ag4683
Full Name	tripartite motif-containing 22
Calculated Molecular Weight	498 aa, 57 kDa
Observed Molecular Weight	50 kDa
GenBank Accession Number	BC035582
Gene Symbol	TRIM22
Gene ID (NCBI)	10346
RRID	AB_2877974
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for TRIM22 antibody 13744-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Commun Identification of DAXX as a restriction factor of SARS-CoV-2 through a CRISPR/Cas9 screen. Authors - Alice Mac Kain View Article
human	WB,IHC	Oncotarget TRIM22 confers poor prognosis and promotes epithelial-mesenchymal transition through regulation of AKT/GSK3β/β-catenin signaling in non-small cell lung cancer. Authors - Li Liu KD Validated View Article
human	WB,IHC,IF	Front Cell Infect Microbiol Constitutive TRIM22 Expression in the Respiratory Tract Confers a Pre-Existing Defence Against Influenza A Virus Infection. Authors - Matthew Charman KD Validated View Article
human	IHC	Immunobiology Shared biomarkers of multi-tissue origin for primary Sjogren's syndrome and their importance in immune microenvironment alterations Authors - Tiantian Cai View Article
human	WB	Cell Biol Int TRIM22 knockdown suppresses chronic myeloid leukemia via inhibiting PI3K/Akt/mTOR signaling pathway. Authors - Liyin Li KD Validated View Article
human	WB	Transl Cancer Res FOXO3/TRIM22 axis abated the antitumor effect of gemcitabine in non-small cell lung cancer via autophagy induction. Authors - Yang Wang KD Validated View Article

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Research Area

TRIM22 Polyclonal antibody

TRIM22 Polyclonal Antibody for IHC, ELISA

Validation Data Gallery

IHC staining of human lung cancer using 13744-1-AP

IHC staining of human lung cancer using 13744-1-AP

IHC staining of human lung cancer using 13744-1-AP

IHC staining of human lung cancer using 13744-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Identification of DAXX as a restriction factor of SARS-CoV-2 through a CRISPR/Cas9 screen.

TRIM22 confers poor prognosis and promotes epithelial-mesenchymal transition through regulation of AKT/GSK3β/β-catenin signaling in non-small cell lung cancer.

Constitutive TRIM22 Expression in the Respiratory Tract Confers a Pre-Existing Defence Against Influenza A Virus Infection.

Shared biomarkers of multi-tissue origin for primary Sjogren's syndrome and their importance in immune microenvironment alterations

TRIM22 knockdown suppresses chronic myeloid leukemia via inhibiting PI3K/Akt/mTOR signaling pathway.

FOXO3/TRIM22 axis abated the antitumor effect of gemcitabine in non-small cell lung cancer via autophagy induction.