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TRIM9 Monoclonal antibody

TRIM9 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

1D2B10

Cat no : 67268-1-Ig

Synonyms

KIAA0282, RING finger protein 91, RNF91, SPRING, TRIM9, tripartite motif containing 9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRIM9 Monoclonal antibody (67268-1-Ig)

    WB analysis using 67268-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67268-1-Ig (TRIM9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat brain tissue using TRIM9 Monoclonal antibody (67268-1-Ig)

    IHC staining of rat brain using 67268-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67268-1-Ig (TRIM9 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using TRIM9 Monoclonal antibody (67268-1-Ig)

    IHC staining of rat brain using 67268-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67268-1-Ig (TRIM9 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat cerebellum tissue using TRIM9 Monoclonal antibody (67268-1-Ig)

    IHC staining of rat cerebellum using 67268-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67268-1-Ig (TRIM9 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat cerebellum tissue using TRIM9 Monoclonal antibody (67268-1-Ig)

    IHC staining of rat cerebellum using 67268-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67268-1-Ig (TRIM9 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inpig brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inrat brain tissue, rat cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67268-1-Ig targets TRIM9 in WB, IHC,ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TRIM9 fusion protein Ag27856
Full Name tripartite motif-containing 9
Calculated Molecular Weight 80 kDa
Observed Molecular Weight 90 kDa, 79 kDa, 61 kDa
GenBank Accession NumberBC013414
Gene Symbol TRIM9
Gene ID (NCBI) 114088
RRIDAB_2882538
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRIM9(E3 ubiquitin-protein ligase TRIM9) is also named as RNF91 and belongs to the TRIM/RBCC family. TRIM9 protein is a brain-specific E3 ubiquitin ligase. Importantly, TRIM9 is present in LBs found in DLB and PD, suggesting that TRIM9 not only plays roles in the regulation of neuronal functions, but also participates in the formation or breakdown of abnormal inclusions through its ligase activity and besides the striatum and hippocampus, the highest expression of TRIM9 protein is seen in the frontal, temporal and occipital cortices through the western blot (PMID:20085810). It has been reported that TRIM9 is highly expressed in the cerebral cortex in mouse and human brains and that TRIM9 is decreased in damaged brains in patients who have Parkinson disease and dementia with Lewy bodies(PMID:22337885). It has 3 isoforms with molecular mass of 79, 90 and 61 kDa.

Protocols

Product Specific Protocols
WB protocol for TRIM9 antibody 67268-1-IgDownload protocol
IHC protocol for TRIM9 antibody 67268-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols