|Positive WB detected in||HeLa cells, MCF-7 cells, Jurkat cells, mouse heart tissue, rat heart tissue|
|Positive IHC detected in||human testis tissue, human breast cancer tissue, mouse testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Positive FC detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:3000-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:300-1:1200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11117-1-AP targets TXNRD1 in WB, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||TXNRD1 fusion protein Ag1618|
|Full Name||thioredoxin reductase 1|
|Calculated molecular weight||55 kDa|
|Observed molecular weight||55 kDa|
|GenBank accession number||BC018122|
|Gene ID (NCBI)||7296|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Thioredoxin reductase-1 (TXNRD1) is uniquely capable of utilizing electrons from NADPH to recover the reduced state of TRX1. Interestingly, TXNRD1 is upregulated in many human malignancies and promotes cancer progression, and attenuation of TXNRD1 levels effectively suppresses the growth of tumor cells (PMID: 31384178). TXNRD1 is also upregulated in many human malignancies and functions as a prognostic factor for many tumors, such as oral squamous cell carcinomas, lung cancer, breast cancer, and astrocytomas. TXNRD1 protein levels were analyzed by inmmunoblotting. Bands of ~55 kDa for TXNRD1 were observed. IHC analysis suggested TXNRD1 was mainly located in the cytoplasm. (PMID: 28536696, PMID: 20584310)
|Product Specific Protocols|
|WB protocol for TXNRD1 antibody 11117-1-AP||Download protocol|
|IHC protocol for TXNRD1 antibody 11117-1-AP||Download protocol|
|IF protocol for TXNRD1 antibody 11117-1-AP||Download protocol|
|FC protocol for TXNRD1 antibody 11117-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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