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UBA6 Polyclonal antibody

UBA6 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 13211-1-AP

Synonyms

E1 L2, Monocyte protein 4, MOP 4, MOP4, UBA6, UBE1L2, Ubiquitin activating enzyme 6



Tested Applications

Positive WB detected inHEK-293 cells, human brain tissue
Positive IF detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13211-1-AP targets UBA6 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBA6 fusion protein Ag4000
Full Name ubiquitin-like modifier activating enzyme 6
Calculated molecular weight 1052 aa, 118 kDa
Observed molecular weight 118 kDa
GenBank accession numberBC031637
Gene symbol UBA6
Gene ID (NCBI) 55236
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UBA6(Ubiquitin-like modifier-activating enzyme 6) is also named as MOP4, UBE1L2 and belongs to the ubiquitin-activating E1 family. It probably acts as a single molecule like UBE1 and not as a heterodimeric E1 complex, as formed by AOS1/Uba2 and APP-BP1/Uba3. UBA6 is even more restricted in distribution because it is only found in vertebrates but not in lower organisms so that it might have a specialized role in tissues of higher organisms, and in particular, in the testis, where it is expressed by far most abundantly.(PMID:17580310). It has 4 isoforms produced by alternative splicing. This antibody recognizes the full-length(118 kda) and the truncated isoform CRA_b (60-70 kDa) of UBA6 (NCBI).

Protocols

Product Specific Protocols
WB protocol for UBA6 antibody 13211-1-APDownload protocol
IF protocol for UBA6 antibody 13211-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols