UBC12 Polyclonal antibody

UBC12 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14520-1-AP

Synonyms

hUbc12, NEDD8 carrier protein, NEDD8 conjugating enzyme Ubc12, NEDD8 protein ligase, UBC RS2, UBC12, UBE2M



Tested Applications

Positive WB detected inHEK-293T cells, L02 cells, SMMC-7721 cells, Ramos cells
Positive IP detected inL02 cells
Positive IHC detected inmouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inL02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14520-1-AP targets UBC12 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBC12 fusion protein Ag5984
Full Name ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast)
Calculated molecular weight 21 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC058924
Gene symbol UBE2M
Gene ID (NCBI) 9040
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for UBC12 antibody 14520-1-APDownload protocol
IHC protocol for UBC12 antibody 14520-1-APDownload protocol
IF protocol for UBC12 antibody 14520-1-APDownload protocol
IP protocol for UBC12 antibody 14520-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochem Biophys Res Commun

Disruption of protein neddylation with MLN4924 attenuates paclitaxel-induced apoptosis and microtubule polymerization in ovarian cancer cells.

Authors - Xiaoling Hong
humanWB

Cancer Biol Med

CUL4 E3 ligase regulates the proliferation and apoptosis of lung squamous cell carcinoma and small cell lung carcinoma.

Authors - Ting Li
humanWB,IF

Nat Commun

A potent small-molecule inhibitor of the DCN1-UBC12 interaction that selectively blocks cullin 3 neddylation.

Authors - Haibin Zhou

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Maria (Verified Customer) (02-06-2018)

I tested a free sample of this antibody. No non-specific bands. Load 40ug of protein for a well (15 well comb) I wish I could use a higher dilution of the antibody.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:250-1:500
  • Cell Tissue Type: EpH4 mammary gland cell line
UBC12 Antibody Western Blot, validation (1:250-1:500 dilution) in EpH4 mammary gland cell line (Cat no:14520-1-AP)