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  • KD/KO Validated

UQCRC1 Polyclonal antibody

UQCRC1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 21705-1-AP

Synonyms

Complex III subunit 1, Core protein I, D3S3191, QCR1, UQCR1, UQCRC1



Tested Applications

Positive WB detected inhuman brain tissue, mouse large intestine tissue, mouse skeletal muscle tissue, HeLa cells, mouse liver tissue, MCF-7 cells, L02 cells, mouse spleen tissue, mouse brain tissue, human skeletal muscle tissue, HepG2 cells, human heart tissue, mouse kidney tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman heart tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21705-1-AP is UQCRC1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UQCRC1 fusion protein Ag16322
Full Name ubiquinol-cytochrome c reductase core protein I
Calculated molecular weight 480 aa, 53 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC009586
Gene symbol UQCRC1
Gene ID (NCBI) 7384
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UQCRC1(Cytochrome b-c1 complex subunit 1, mitochondrial), also named as QCR1 and UQCR1, is a ubunit of the cytochrome bc1 complex (complex III) of the mitochondrial respiratory chain, may mediate the formation of the complex between cytochromes c and c1. The gene encodes a 53 kDa protein with a 34 amino acids transit peptide. It is associated with apoptosis or inhibition of cancer cell growth.

Protocols

Product Specific Protocols
WB protocol for UQCRC1 antibody 21705-1-APDownload protocol
IHC protocol for UQCRC1 antibody 21705-1-APDownload protocol
IF protocol for UQCRC1 antibody 21705-1-APDownload protocol
IP protocol for UQCRC1 antibody 21705-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

The SIAH2-NRF1 axis spatially regulates tumor microenvironment remodeling for tumor progression.

Authors - Biao Ma
humanWB

Mol Cell Biol

Iron supply via NCOA4-mediated ferritin degradation maintains mitochondrial functions.

Authors - Motoki Fujimaki
humanWB

Dis Markers

CYC1 Predicts Poor Prognosis in Patients with Breast Cancer.

Authors - Yingyan Han
humanWB,IF

DNA Cell Biol

Inhibition of the ox-LDL-Induced Pyroptosis by FGF21 of Human Umbilical Vein Endothelial Cells Through the TET2-UQCRC1-ROS Pathway.

Authors - Jiao-Jiao Chen
  • KD Validated
humanWB

Acta Pharmacol Sin

Benzoylaconine induces mitochondrial biogenesis in mice via activating AMPK signaling cascade.

Authors - Xiao-Hong Deng
mouse, humanWB

PLoS One

Tetramethylpyrazine ameliorates high glucose-induced endothelial dysfunction by increasing mitochondrial biogenesis.

Authors - Qiong Xu