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  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ZAK Recombinant antibody (81161-3-RR)

    IHC staining of human liver cancer using 81161-3-RR

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81161-3-RR (ZAK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ZAK Recombinant antibody (81161-3-RR)

    IHC staining of human liver cancer using 81161-3-RR

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81161-3-RR (ZAK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ZAK Recombinant antibody (81161-3-RR)

    IF Staining of HepG2 using 81161-3-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using ZAK antibody (81161-3-RR, Clone: 250559E1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 81161-3-RR

    Affinity and Kinetic Characterization of 81161-3-RR

    Biolayer interferometry (BLl) kinetic assays of 81161-3-RR against Human ZAK were performed. The affinity constant is 2.57 nM.

ZAK Recombinant antibody

ZAK Recombinant Antibody for IHC, IF/ICC, ELISA

Cat No. 81161-3-RR
Clone No.250559E1

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Synonyms

AZK, EC:2.7.11.25, HCCS 4, HCCS-4, Leucine zipper- and sterile alpha motif-containing kinase

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81161-3-RR targets ZAK in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ZAK fusion protein Ag30599 Predict reactive species
Full Name sterile alpha motif and leucine zipper containing kinase AZK
Calculated Molecular Weight 91 kDa
GenBank Accession NumberBC001401
Gene Symbol ZAK
Gene ID (NCBI) 51776
Conjugate Unconjugated
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9NYL2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZAK(sterile-alpha motif and leucine zipper containing kinase AZK) is also named as MLTK, MAPKKK, mlklak, MLK7, AZK, MLT, MRK, HCCS-4, MAP3K20 and belongs to the MAPKKK family. It is a mitogen-activated protein kinase kinase kinase (MAP3K) that activates the stress-activated protein kinase/c-jun N-terminal kinase pathway and activates NF-kappaB. ZAK contributes to regulation of DNA damage checkpoints through a p38 gamma-independent pathway. This protein has 3 isoforms produced by alternative splicing with the MW of 91 kDa (ZAK alpha), 51 kDa (ZAK beta) and 35 kDa.

Protocols

Product Specific Protocols
IHC protocol for ZAK antibody 81161-3-RRDownload protocol
IF protocol for ZAK antibody 81161-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols