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ZG16 Polyclonal antibody

ZG16 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17397-1-AP

Synonyms

hZG16, JCLN, JCLN1, Secretory lectin ZG16, ZG16, ZG16A, Zymogen granule protein 16

Validation Data Gallery

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  • Western Blot (WB) analysis of human colon tissue using ZG16 Polyclonal antibody (17397-1-AP)

    WB analysis of human colon using 17397-1-AP

    human colon tissue were subjected to SDS PAGE followed by western blot with 17397-1-AP (ZG16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human colon tissue using ZG16 Polyclonal antibody (17397-1-AP)

    WB analysis of human colon using 17397-1-AP

    human colon tissue were subjected to SDS PAGE followed by western blot with 17397-1-AP (ZG16 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ZG16 Polyclonal antibody (17397-1-AP)

    WB analysis using 17397-1-AP

    mouse colon tissue lysates were subjected to SDS PAGE followed by western blot with 17397-1-AP (ZG16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using ZG16 Polyclonal antibody (17397-1-AP)

    IHC staining of mouse pancreas using 17397-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 17397-1-AP (ZG16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using ZG16 Polyclonal antibody (17397-1-AP)

    IHC staining of mouse pancreas using 17397-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 17397-1-AP (ZG16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using ZG16 Polyclonal antibody (17397-1-AP)

    IHC staining of mouse small intestine using 17397-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 17397-1-AP (ZG16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using ZG16 Polyclonal antibody (17397-1-AP)

    IHC staining of mouse colon using 17397-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 17397-1-AP (ZG16 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using ZG16 Polyclonal antibody (17397-1-AP)

    IHC staining of mouse colon using 17397-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 17397-1-AP (ZG16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using ZG16 Polyclonal antibody (17397-1-AP)

    IHC staining of human pancreas using 17397-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 17397-1-AP (ZG16 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using ZG16 Polyclonal antibody (17397-1-AP)

    IHC staining of human pancreas using 17397-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 17397-1-AP (ZG16 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of mouse small intestine tissue using ZG16 Polyclonal antibody (17397-1-AP)

    IF Staining of mouse small intestine using 17397-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using ZG16 antibody (17397-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman colon tissue, mouse colon tissue
Positive IHC detected inmouse pancreas tissue, human pancreas tissue, mouse colon tissue, mouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse small intestine tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:600
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

17397-1-AP targets ZG16 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZG16 fusion protein Ag11332
Full Name zymogen granule protein 16 homolog (rat)
Calculated Molecular Weight 167 aa, 18 kDa
Observed Molecular Weight 16 kDa
GenBank Accession NumberBC029149
Gene Symbol ZG16
Gene ID (NCBI) 653808
RRIDAB_1939810
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ZG16 antibody 17397-1-APDownload protocol
IHC protocol for ZG16 antibody 17397-1-APDownload protocol
IF protocol for ZG16 antibody 17397-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

Co-transplantation of autologous Treg cells in a cell therapy for Parkinson's disease

Authors - Tae-Yoon Park
View Article