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CD206 Monoklonaler Antikörper
CD206 Monoklonal Antikörper für IF, IHC, IP, WB, ELISA
Wirt / Isotyp
Maus / IgG2a
Getestete Reaktivität
human und mehr (1)
Anwendung
WB, IP, IHC, IF, FC, ELISA
Konjugation
Unkonjugiert
CloneNo.
2A6A10
Kat-Nr. : 60143-1-Ig
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | humanes Plazenta-Gewebe, humanes Lebergewebe |
| Erfolgreiche IP | humanes Plazenta-Gewebe |
| Erfolgreiche Detektion in IHC | humanes Lungenkarzinomgewebe, humanes Lebergewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
| Erfolgreiche Detektion in IF | humanes Lungenkarzinomgewebe |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunhistochemie (IHC) | IHC : 1:10000-1:40000 |
| Immunfluoreszenz (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 1 publications below |
| WB | See 24 publications below |
| IHC | See 43 publications below |
| IF | See 65 publications below |
| FC | See 4 publications below |
Produktinformation
60143-1-Ig bindet in WB, IP, IHC, IF, FC, ELISA CD206 und zeigt Reaktivität mit human
| Getestete Reaktivität | human |
| In Publikationen genannte Reaktivität | human, Hausschwein |
| Wirt / Isotyp | Maus / IgG2a |
| Klonalität | Monoklonal |
| Typ | Antikörper |
| Immunogen | Peptid |
| Vollständiger Name | mannose receptor, C type 1 |
| Berechnetes Molekulargewicht | 166 kDa |
| Beobachtetes Molekulargewicht | 170 kDa |
| GenBank-Zugangsnummer | NM_002438 |
| Gene symbol | MRC1 |
| Gene ID (NCBI) | 4360 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Protein-A-Reinigung |
| Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
CD206, also named as MMR, CLEC13D and MRC1, is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. CD206 has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment. CD206 is a 170 kDa transmembrane protein which contains 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail. It is expressed on most tissue macrophages, in vitro derived dendritic cells, lymphatic and sinusoidal endothelia. This antibody recognizes the intracellular carboxy-terminal part of CD206 and MRC1L1.
Protokolle
| Produktspezifische Protokolle | |
|---|---|
| WB protocol for CD206 antibody 60143-1-Ig | Protokoll herunterladen |
| IHC protocol for CD206 antibody 60143-1-Ig | Protokoll herunterladen |
| IF protocol for CD206 antibody 60143-1-Ig | Protokoll herunterladen |
| IP protocol for CD206 antibody 60143-1-Ig | Protokoll herunterladen |
| Standard-Protokolle | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
ACS Nano Tailoring Chemoimmunostimulant Bioscaffolds for Inhibiting Tumor Growth and Metastasis after Incomplete Microwave Ablation. | ||
ACS Nano Smart Liposomal Nanocarrier Enhanced the Treatment of Ischemic Stroke through Neutrophil Extracellular Traps and Cyclic Guanosine Monophosphate-Adenosine Monophosphate Synthase-Stimulator of Interferon Genes (cGAS-STING) Pathway Inhibition of Ischemic Penumbra | ||
Cancer Commun (Lond) Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer | ||
Nat Commun P-selectin axis plays a key role in microglia immunophenotype and glioblastoma progression. | ||
Nat Commun Mitochondrial fission induces immunoescape in solid tumors through decreasing MHC-I surface expression. | ||
Mater Today Bio The spatial form periosteal-bone complex promotes bone regeneration by coordinating macrophage polarization and osteogenic-angiogenic events |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Kenzo (Verified Customer) (03-07-2023) | Works great with minimal background for immunofluorescence
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