CD206 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

CD206 Monoklonal Antikörper für WB, IHC, IF-P, IP, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(218)

CloneNo.

2A6A10

Rezensionen (1)

Kat-Nr. : 60143-1-Ig

Synonyme

hMR, C-type lectin domain family 13 member D-like, C-type lectin domain family 13 member D, CLEC13DL, CLEC13D

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  WB analysis of human placenta using 60143-1-Ig

  human placenta tissue was subjected to SDS PAGE followed by western blot with 60143-1-Ig (CD206 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 60143-1-Ig

  human liver tissue were subjected to SDS PAGE followed by western blot with 60143-1-Ig (CD206 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of human placenta using 60143-1-Ig

  IP result of anti-CD206 (IP:60143-1-Ig, 5ug; Detection:60143-1-Ig 1:300) with human placenta tissue lysate 1520ug.

• 

  IHC staining of human lung cancer using 60143-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60143-1-Ig (CD206 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 60143-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60143-1-Ig (CD206 antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 60143-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60143-1-Ig (CD206 antibody) at dilution of 1:80000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 60143-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60143-1-Ig (CD206 antibody) at dilution of 1:80000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 60143-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60143-1-Ig (CD206 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 60143-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60143-1-Ig (CD206 antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human lung cancer using 60143-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CD206 antibody (60143-1-Ig, Clone: 2A6A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human lung cancer using 60143-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CD206 antibody (60143-1-Ig, Clone: 2A6A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe, humanes Lebergewebe
• Erfolgreiche IP: humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Lebergewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Lungenkarzinomgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:10000-1:40000
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 53 publications below
• IHC: See 69 publications below
• IF: See 130 publications below
• FC: See 5 publications below

Produktinformation

60143-1-Ig bindet in WB, IHC, IF-P, IP, ELISA CD206 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: mannose receptor, C type 1
• Berechnetes Molekulargewicht: 166 kDa
• Beobachtetes Molekulargewicht: 170 kDa
• GenBank-Zugangsnummer: NM_002438
• Gene symbol: CD206
• Gene ID (NCBI): 4360
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Background

CD206 (macrophage mannose receptor 1) is a lectin-type endocytic receptor expressed on selected macrophages, dendritic cells, and non-vascular endothelium and plays a role in antigen processing and presentation, phagocytosis, and intracellular signaling.

1. What is the molecular weight of CD206?

The molecular size of full-length CD206 is 170-180 kDa, depending on the exact tissue-specific glycosylation pattern (PMID: 19427834). Additionally, CD206 can be cleaved off and a soluble form (sMR) lacking the tail, with a slightly lower molecular weight, can be released to the cell medium (PMID: 9722572).

2. What is the subcellular localization of CD206?

CD206 is a type I membrane protein composed of a large extracellular multidomain, a transmembrane domain, and a short cytoplasmic tail. It is present at the plasma membrane and in endosomes, as CD206 undergoes constant recycling between the plasma membrane and endosomal compartment.

3. Is CD206 post-translationally modified?

CD206 undergoes quite extensive post-translational modifications, predominantly N-linked glycosylation that affects ligand binding recognition and affinity (PMID: 22966131).

4. Can CD206 marker be used as a marker of M2 macrophages?

The activation of macrophages with various stimuli leads to their polarization into classical (M1) or alternatively activated (M2) subtypes spectrums and both subtypes differ in their regulatory and effector functions (PMID: 24669294). Pathogens and IFN-γ promote M1 polarization, while IL-4 released during parasite infections and allergen response promotes M2 polarization. Classically, the markers of M2 macrophages include CD206, as well as arginase-1 (ARG1; https://www.ptglab.com/products/ARG1-Antibody-16001-1-AP.htm), CD163 (https://www.ptglab.com/products/CD163-Antibody-16646-1-AP.htm), and thrombospondin 1 (TSP1/ THBS1; https://www.ptglab.com/products/TSP1-Antibody-18304-1-AP.htm).

5. How can you polarize macrophages into M2 direction?

One of the most commonly used methods is stimulation by the addition of IL-4 cytokine. We recommend using our animal-free human IL-4 (https://www.ptglab.com/products/recombinant-human-il-4.htm).

Publikationen

Species	Application	Title
	IF	Adv Mater; Photosensitive Biomimetic Nanomedicine-Mediated Recombination of Adipose Microenvironments for Antiobesity Therapy; Authors - Mingming Song
	IF	Nat Neurosci; A phenotypic screening platform for identifying chemical modulators of astrocyte reactivity; Authors - Benjamin L L Clayton
	IF	Bioact Mater; Profibrogenic macrophage-targeted delivery of mitochondrial protector via exosome formula for alleviating pulmonary fibrosis; Authors - Wei Zhang
	IF	ACS Nano; Tailoring Chemoimmunostimulant Bioscaffolds for Inhibiting Tumor Growth and Metastasis after Incomplete Microwave Ablation.; Authors - Yuting Shen
	IF	ACS Nano; Smart Liposomal Nanocarrier Enhanced the Treatment of Ischemic Stroke through Neutrophil Extracellular Traps and Cyclic Guanosine Monophosphate-Adenosine Monophosphate Synthase-Stimulator of Interferon Genes (cGAS-STING) Pathway Inhibition of Ischemic Penumbra; Authors - Shanbo Sun
human	IHC,IF	Cancer Commun (Lond); Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer; Authors - Xingbin Hu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kenzo (Verified Customer) (03-07-2023)

Works great with minimal background for immunofluorescence

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Optic nerve