• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-4 protein

Activity

0.07-0.4 ng/mL EC50

Specific Activity

5.24x10^6 IU/mg calibrated against IL-4 reference standard (NIBSC cat no: 88/656). Reference use only. Specific activity is not routinely tested

Species Reactivity

human,mouse

Purity

>95%

Cat no : HZ-1004

Synonyms

B cell stimulatory factor 1, Binetrakin, BSF 1, BSF1, IL 4, IL4, IL-4, interleukin 4, Pitrakinra



Technical Specifications

GeneID 3565
Species Human
Expression HEK293
Activity 0.07-0.4 ng/mL EC50
Specific Activity5.24x10^6 IU/mg calibrated against IL-4 reference standard (NIBSC cat no: 88/656). Reference use only. Specific activity is not routinely tested
Purity >95%
Endotoxin<1 EU/µg
Accession NumberP05112
Molecular Mass 14 and 19 kDa, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

Interleukin-4 (IL-4), a member of the α-helical cytokine family, is produced by activated CD4+ T cells, basophils, and mast cells. It promotes the proliferation and differentiation of antigen presenting cells. IL4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival (PMID: 24489573;3049907;21663408)

Synonyms

B cell stimulatory factor 1, Binetrakin, BSF 1, BSF1, IL 4, IL4, IL-4, interleukin 4, Pitrakinra

Publications

SpeciesTitle
human

J Exp Med

Inherited TNFSF9 deficiency causes broad Epstein-Barr virus infection with EBV+ smooth muscle tumors.

Authors - Benjamin Fournier

Biomaterials

In situ repurposing of dendritic cells with CRISPR/Cas9-based nanomedicine to induce transplant tolerance.

Authors - Yue Zhang

J Neuroinflammation

Monocarboxylate transporter 1 promotes classical microglial activation and pro-inflammatory effect via 6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 3.

Authors - Liang Kong
human

J Virol

HIV-1 Tat protein induces PD-L1 (B7-H1) expression on dendritic cells through tumor necrosis factor alpha- and toll-like receptor 4-mediated mechanisms.

Authors - RémiPlanès
human

Immunol Cell Biol

Regulation of human Th9 differentiation by type I interferons and IL-21.

Authors - Michael TWong
human

J Biol Chem

Cysteine-rich domain 1 of CD40 mediates receptor self-assembly.

Authors - Cristian RSmulski