• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-4 protein

Activity

0.07-0.4 ng/mL EC50

Specific Activity

5.24x10^6 IU/mg calibrated against IL-4 reference standard (NIBSC cat no: 88/656). Reference use only. Specific activity is not routinely tested

Species Reactivity

human,mouse

Purity

>95%

Cat no : HZ-1004

Synonyms

B cell stimulatory factor 1, Binetrakin, BSF 1, BSF1, IL 4, IL4, IL-4, interleukin 4, Pitrakinra



Technical Specifications

GeneID 3565
Species Human
Expression HEK293
Activity 0.07-0.4 ng/mL EC50
Specific Activity5.24x10^6 IU/mg calibrated against IL-4 reference standard (NIBSC cat no: 88/656). Reference use only. Specific activity is not routinely tested
Purity >95%
Endotoxin<1 EU/µg
Accession NumberP05112
Molecular Mass 14 and 19 kDa, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

Interleukin-4 (IL-4), a member of the α-helical cytokine family, is produced by activated CD4+ T cells, basophils, and mast cells. It promotes the proliferation and differentiation of antigen presenting cells. IL4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival (PMID: 24489573;3049907;21663408)

Synonyms

B cell stimulatory factor 1, Binetrakin, BSF 1, BSF1, IL 4, IL4, IL-4, interleukin 4, Pitrakinra

Publications

SpeciesTitle
mouse

Pharm Biol

β-Sitosterol modulates macrophage polarization and attenuates rheumatoid inflammation in mice.

Authors - Rui Liu
human,mouse

ArXiv

Darwin's Neural Network: AI-based Strategies for Rapid and Scalable Cell and Coronavirus Screening.

Authors - Sang Won Lee
human

J Toxicol Sci

Human induced pluripotent stem cell-derived mast cells useful for in vitro mast cell activation assay exhibiting phenotypes and morphological characteristics of human mast cells.

Authors - Tatsuya Ikuno
human

J Oncol

circRNA TCFL5 Promote Esophageal Cancer Progression by Modulating M2 Macrophage Polarization via the miR-543-FMNL2 Axis.

Authors - Chengbin Lin
human

Clin Exp Pharmacol Physiol

A tumour-promoting role of Th9 cells in hepatocellular carcinoma through CCL20 and STAT3 pathways.

Authors - HongwuTan
human

J Gen Virol

iPS cell serves as a source of dendritic cells for in vitro dengue virus infection model.

Authors - Dao Huy Manh