Anticorps Polyclonal de lapin anti-Glypican 3

Glypican 3 Polyclonal Antibody for ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 11145-1-AP

Synonymes

DGSX, Glypican 3, GPC3, GTR2 2, Intestinal protein OCI 5, MXR7, OCI 5, OCI5, SDYS, SGB, SGBS, SGBS1

---

Galerie de données de validation

• 

---

Applications testées

Dilution recommandée

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

11145-1-AP cible Glypican 3 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Glypican 3 Protéine recombinante Ag1433
• Nom complet: glypican 3
• Masse moléculaire calculée: 580 aa, 66 kDa
• Poids moléculaire observé: 66 kDa
• Numéro d’acquisition GenBank: BC035972
• Symbole du gène: GPC3
• Identification du gène (NCBI): 2719
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Glypicans (GPCs) are a family of glycosylphosphatidylinositol (GPI)-anchored heparan sulphate proteoglycans (HSPGs) that may play a role in the control of cell division and growth regulation. In mammals, there are six GPCs (GPC1 to GPC6), all of which have a similar core-protein size of approx. 60 kDa and the clustering of glycosaminoglycan attachment site near the C-terminus. They are tethered to the cell surface by GPI linkages, which can be cleaved by endogenous phospholipases, thus releasing the protein. Glypican 3 (GPC3) is highly expressed in many tissues during development and plays an important role in the regulation of embryonic growth (PMID: 22467855). Loss-of-function mutations of GPC3 result in the Simpson-Golabi-Behmel overgrowth syndrome (SGBS), and Gpc-3 null mice display developmental overgrowth (PMID: 8589713; 18477453). In hepatocellular carcinoma (HCC), the overexpression of GPC3 has been demonstrated to be a reliable diagnostic indicator (PMID: 19212669; 22706665).

Publications

Species	Application	Title
human	WB	Aging (Albany NY); Kinsenoside ameliorates intervertebral disc degeneration through the activation of AKT-ERK1/2-Nrf2 signaling pathway.; Authors - Yanqiu Wang
rat	WB	Cell Physiol Biochem; N-Cadherin Maintains the Healthy Biology of Nucleus Pulposus Cells under High-Magnitude Compression.; Authors - Zhenyu Wang
	WB	Mol Med Rep; Silencing of the glypican-3 gene affects the biological behavior of human hepatocellular carcinoma cells.; Authors - Xin-Hui Qi