Integrin alpha 4 Recombinant monoclonal antibody

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• Integrin alpha 4 Recombinant monoclonal antibody (87464-1-RR)

• 

  WB analysis using 87464-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87464-1-RR (ITA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity of 87464-1-RR

  Biolayer interferometry (BLl) kinetic assays of 87464-1-RR against Mouse Integrin alpha 4 were performed. The affinity constant is 0.324 nM.

Integrin alpha 4 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87464-1-RR

Clone No.252738G5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

CD49d, Itga4, CD49 antigen-like family member D, Integrin alpha-4, Integrin alpha-IV

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Tested Applications

• Positive WB detected in: Jurkat cells, THP-1 cells, mouse spleen tissue, rat thymus tissue, rat spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87464-1-RR targets Integrin alpha 4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg10472

  Product name: Recombinant mouse ITA4 protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-Twin-Strep-Tag

  Domain: 10011443 of

  Sequence: \

• Full Name: integrin alpha 4
• Observed Molecular Weight: 70 kDa, 140-150 kDa
• Gene Symbol: Itga4
• Gene ID (NCBI): 16401
• RRID: AB_3745563
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q00651
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Integrin alpha 4 (ITGA4), also named CD49D, belongs to the integrin alpha chain family. ITGA4 is a receptor for fibronectin, VCAM1, and MADCAM1. ITGA4 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. It recognizes the sequence Q-I-D-S in VCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells, ITGA4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells.