Anticorps Polyclonal de lapin anti-ANT1/2

ANT1/2 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 15997-1-AP

Synonymes

SLC25A4, AAC1, ADP,ATP carrier protein 1, ADP/ATP translocase 1, ANT

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Galerie de données de validation

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  WB analysis of human heart using 15997-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 15997-1-AP (ANT1/2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 15997-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 15997-1-AP (ANT1/2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 15997-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15997-1-AP (ANT1/2antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15997-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15997-1-AP (ANT1/2antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 15997-1-AP

  IP result of anti-ANT1/2 (IP:15997-1-AP, 4ug; Detection:15997-1-AP 1:500) with mouse heart tissue lysate 4000ug.

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Applications testées

• Résultats positifs en WB: tissu cardiaque humain, cellules MCF-7, tissu cérébral de souris, tissu de muscle squelettique de souris
• Résultats positifs en IP: tissu cardiaque de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• CoIP: See 1 publications below

Informations sur le produit

15997-1-AP cible ANT1/2 dans les applications de WB, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ANT1/2 Protéine recombinante Ag7787
• Nom complet: solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 4
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC008664
• Symbole du gène: SLC25A4
• Identification du gène (NCBI): 291
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Adenine nucleotide translocators (ANTs) are mitochondrial carrier proteins that exchange ATP and ADP between the cytoplasm and the mitochondrial matrix. Four isoforms of ANT were identified: ANT1 (encoded by SLC25A4) is highly expressed in different tissues; ANT2 (encoded by SLC25A5) is predominantly expressed in proliferating, regenerating, or undifferentiated cells; ANT3 is ubiquitously expressed; ANT4 is mainly expressed in testis and germ cells. This antibody recognizes both of ANT1 and ANT2.

Publications

Species	Application	Title
pig	WB	J Biol Chem; A new circular RNA-encoded protein BIRC6-236aa inhibits transmissible gastroenteritis virus (TGEV)-induced mitochondrial dysfunction.; Authors - Xiaomin Zhao
mouse	WB	Neurochem Int; Curcumin prevents mitochondrial dysfunction in the brain of the senescence-accelerated mouse-prone 8.; Authors - Eckert Gunter P GP
human	WB	Dev Cell; Deregulation of ER-mitochondria contact formation and mitochondrial calcium homeostasis mediated by VDAC in fragile X syndrome; Authors - Ji Geng
human	WB,CoIP	iScience; MRPL12-ANT3 interaction involves in acute kidney injury via regulating MPTP of tubular epithelial cells; Authors - Xingzhao Ji
mouse	WB	Int J Biol Macromol; Mfn2R364W, Mfn2G176S, and Mfn2H165R mutations drive Charcot-Marie-Tooth type 2A disease by inducing apoptosis and mitochondrial oxidative phosphorylation damage; Authors - Yuanzhu Zhang

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