Anticorps Polyclonal de lapin anti-TSG101

• Phare
• Validé par KD/KO

TSG101 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (3)

Applications

WB, RIP, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

205

Avis (1)

N° de cat : 14497-1-AP

Synonymes

ESCRT I complex subunit TSG101, TSG10, TSG101, tumor susceptibility gene 101, VPS23

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Galerie de données de validation

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  WB analysis using 14497-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14497-1-AP (TSG101 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 14497-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14497-1-AP (TSG101 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14497-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14497-1-AP (TSG101 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14497-1-AP

  IP Result of anti-TSG101 (IP:14497-1-AP, 3ug; Detection:14497-1-AP 1:500) with HeLa cells lysate 1320ug.

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  IHC staining of human colon cancer using 14497-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14497-1-AP (TSG101 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 14497-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14497-1-AP (TSG101 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 14497-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14497-1-AP (TSG101 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 14497-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14497-1-AP (TSG101 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 14497-1-AP

  Immunofluorescent analysis of Hela cells, using TSG101 antibody 14497-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  FC experiment of HeLa using 14497-1-AP

  1X10^6 HeLa cells were stained with 0.2ug TSG101 antibody (14497-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, cellules HeLa
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 191 publications below
• IHC: See 3 publications below
• IF: See 6 publications below
• IP: See 1 publications below
• FC: See 3 publications below
• RIP: See 1 publications below

Informations sur le produit

14497-1-AP cible TSG101 dans les applications de WB, RIP, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, canin, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TSG101 Protéine recombinante Ag5920
• Nom complet: tumor susceptibility gene 101
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC002487
• Symbole du gène: TSG101
• Identification du gène (NCBI): 7251
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TSG101(Tumor susceptibility gene 101 protein) is essential for endosomal sorting, membrane receptor degradation and the final stages of cytokinesis. It plays a crucial role for cell proliferation and cell survival. TSG101 has been identified as a candidate tumor suppressor gene and belongs to the ubiquitin-conjugating enzyme family. TSG101 is a marker for exosome. This protein has 2 isoforms produced by alternative splicing with the molecular mass of 44 and 32 kDa.

Publications

Species	Application	Title
human, mouse	WB, IHC	Nature; Microenvironment-induced PTEN loss by exosomal microRNA primes brain metastasis outgrowth.; Authors - Lin Zhang
human	WB	Cell; Exosome transfer from stromal to breast cancer cells regulates therapy resistance pathways.; Authors - Mirjam C Boelens
human	WB	Cell; Exosome RNA Unshielding Couples Stromal Activation to Pattern Recognition Receptor Signaling in Cancer.; Authors - Barzin Y Nabet
human	WB	Nat Immunol; Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity.; Authors - Li Jianhua J
human	IF	Nat Cell Biol; Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.; Authors - Vincent Mercier ; KD Validated
mouse,human	WB	ACS Nano; Mesenchymal Stem Cell-Derived Extracellular Vesicles Attenuate Mitochondrial Damage and Inflammation by Stabilizing Mitochondrial DNA.; Authors - Meng Zhao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Pasquale (Verified Customer) (11-06-2019)

The antibody recognize the exosomal marker TSG101 as claimed. We tested it by Western blotting in our exosome preps isolated from murine brains and it gave the signal at the correct molecular weight in the fractions that are enriched in exosomes, so it seems to be specific. However, we report here some drawbacks that we have experienced using this antibody (see the file attached):1. There is a strong cross-reactivity of the antibody with the molecular weight marker we use (The Bio-Rad Dual Color Standard, cat. number 1610374)2. The signal over noise ratio is a little low (the background coming from the membrane is pretty high)

• Applications: Western Blot,
• Primary Antibody Dilution: 1 to 1000
• Cell Tissue Type: Murine brain extracellular vesicles fractions

TSG101 Antibody Western Blot, validation (1 to 1000 dilution) in Murine brain extracellular vesicles fractions (Cat no:14497-1-AP)

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