Optimizing Immunoprecipitation of GFP-tagged Proteins in Plant Cell Extracts
Live on August 7th, 2025
9am CDT / 3pm BST / 4pm CEST
Immunoprecipitation (IP) is a powerful technique for isolating and analyzing proteins and their interaction partners (co-IP) from complex biological samples. In plant systems, however, the limited availability of antibodies against endogenous plant proteins often leads researchers to use tagged proteins, with green fluorescent protein (GFP) being one of the most widely used tags due to its versatility and ease of detection.
Speaker
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Dr. Mayank Sharma Scientist at ETH Zurich Dr Sharma will present his optimized protocol for IP of GFP-tagged proteins from crude plant cell extracts, as detailed in Methods in Molecular Biology (PMID: 36107351).
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Dr. Sharma will cover:
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A step-by-step workflow using GFP-Trap for efficient IP from plant cell extracts
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Practical tips and best practices for maximizing pulldown efficiency
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Troubleshooting guidance to help reduce background and improve reproducibility
Attendees will receive:
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In-depth Q&A session with Dr. Sharma
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Certificate of attendance
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Free GFP-Trap sample
A certificate of attendance and a link to watch the webinar will be available to attendees post-event.