TDP-43 Polyclonal antibody

It works really nice for the IF, nice picture with less background

• Applications: Immunofluorescence

I used it at 1:1000 for WB and at 1:200 for IF, and it worked as expected.

• Applications: Western Blot, Immunofluorescence
• Cell Tissue Type: IPSCs derived motoneurons

The antibody shows very nice staining by IF and also works well by WB

• Applications: Western Blot, Immunofluorescence
• Cell Tissue Type: iPSC-derived motor neurons

Used this for checking our in house TDP production quality on WB. We did a lot of tests with other Ab and we trust this one here.

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Mouse

This antibody worked well for our lab.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000

We love the results of this antibody

• Applications: Immunohistochemistry
• Primary Antibody Dilution: TDP43
• Cell Tissue Type: Brain

N/A

• Applications: Cell culture

Worked well for Opal staining of TDP-43 in human brain tissue sections and visualization on confocal

• Applications: Immunohistochemistry
• Cell Tissue Type: Brain section

10µg of protein was loaded and antibody was incubated overnight at 4oC following a total protein stain. The band appeared at the expected size but with two bands, blue bars, with Alpha-tubulin internal control (red band - 66031-1-Ig). Precision plus protein standard ladder #1610373.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: TDP43 Q331K Mouse Brain Lysate

Didn't work well for western blot, had high background

• Applications: Western Blot

We love this item since we start to use it.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:2000-1:8000
• Cell Tissue Type: IHQ for neuroscience

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293, Neuronal stem cells, Mouse tissue

Top notch antibody, single band at predicted molecular weight. Gold standard.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: SH-SY5Y lysate

Good antibody for IF

• Applications: Immunofluorescence

This antibody is one of the most important ones we use in the lab for studying ALS! It works very well and we will continue to use it.

• Applications: Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: iPSC-derived neurons

everything ok

• Applications: Immunofluorescence
• Cell Tissue Type: HeLa

Very good in WB with a band of around 45 kD.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: mouse brain lysate

good for WB and IF

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000

TDP43 in human primary fibroblasts. 10 ug of total protein. Primary ab incubation 16h 4ºC 1:1000 in BSA 3% in PBST. Secondary ab Goat anti-rabbit HRP 1:5000 1h RT incubation.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Primary human fibrosblasts

Works good for ICC in fibroblasts. High nuclear signal. Cells fixed with 4% PFA 15 min. TDP43 ab dilution 1:200 in PBST O/N 4ºC. Donkey anti-rabbit Alexafluor 568 1:500 for 1h RT.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: fibroblast cell

the antibody work prefect for my staining.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: iPSCs

Good product for staining.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Neuron

TDP43 in human primary fibroblasts. Loading: 10 ug in LB with DTT. TGX 4-15% gel. Blocking: 1h 3% BSA in PBST (0.1% tween). Primary ab incubation 16h 4ºC 1:1000 in BSA 3% in PBST (0.1% Tween) Detection: Goat anti-rabbit HRP + ECL+.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human Primary Fibroblasts

Good ICC in human primary fibroblats. Small amount of signal in cytoplasm in UT and control cells. High nuclear signal. Fibroblats fixed with 4% PFA 15 min. TDP43 ab dilution 1:100 in PBST (0.1% triton) + 10% DS O/N 4ºC. Donkey anti-rabbit alexa fluor 555 1:500 in PBST+10%DS 1h RT.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human fibroblasts

Produces good IF images with minimal background.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: HEK293T

Works very well

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:300

Works well in both western blotting and Immunofluorescence. Nice clear images obtained with IF.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:2500, 1:100
• Cell Tissue Type: HEK293T

It has great specificity over wide range of species.

• Applications: Western Blot,

Great N-terminal TDP-43 antibody, works really well for immunohistochemistry, immunofluorescence and western blot from mouse brain tissue. Unfortunately, I have not been able to get it to work for precipitation in mouse brain.

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence,
• Primary Antibody Dilution: IHC and WB: 1:1000. IF: 1:400
• Cell Tissue Type: Mouse brain tissue

I used it for ICC and it had low signal and high background with following (1/500, 1/300, 1/100,1/50) dilutions. Cell were fixed by 4% PFA.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1/500-1/300-1/100- 1/50
• Cell Tissue Type: NSC-34- Primary Hippocampal Neuron Culture (Rat P0-P1 Pups)

Antibody worked well. My lab has used the antibody in various experiments for quantifications, many of which were included in published works.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Mouse

Doxycycline-inducible SHSY5Y show GFP-TDP-43 expression upon dox addition.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: SHSY5Y

Excellent for both immunoblot and immunocytochemistry. Strong signal and low background in both cases. Single band in control cells for immunoblot.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:1000 (IB), 1:500 (ICC)
• Cell Tissue Type: Neuroblastoma, iPSC-derived neurons

Good antibody to show mislocalisation in transgenic ALS disease mouse model tissue culture. Needs a high concentration to show up for microscopy but very strong and clear signal.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Mouse Spinal Cord/Brain slices

• Applications: Western Blot,
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: HEK-293 cells

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Brain tissue