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  • Western Blot (WB) analysis of various lysates using OPCML Polyclonal antibody (17962-1-AP)

    WB analysis using 17962-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17962-1-AP (OPCML antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

OPCML Polyclonal antibody

OPCML Polyclonal Antibody for WB, ELISA
Cat No. 17962-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

IgLON family member 1, IGLON1, OBCAM, OPCM, OPCML

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inmouse pancreas tissue, rat pancreas tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17962-1-AP targets OPCML in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OPCML fusion protein Ag12560 Predict reactive species
Full Name opioid binding protein/cell adhesion molecule-like
Calculated Molecular Weight 345 aa, 38 kDa
Observed Molecular Weight45 kDa
GenBank Accession NumberBC074773
Gene Symbol OPCML
Gene ID (NCBI) 4978
RRIDAB_2935445
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14982
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OPCML, also called opioid-binding cell adhesion molecule (OBCAM), belongs to the IgLON limbic system-associated membrane protein (LSAMP, OPCML/OBCAM, neurotrimin) family of IgG domain containing glycosylphosphatidylinositol-anchored cell adhesion molecules. Originally isolated from brain, OPCMLis also expressed in stomach, ovary, heart, placenta, testis, kidney, liver, pancreas and colon. OPCML has been identified as a tumour suppressor, which inhibited tumor growth in some cancers including ovarian cancer, lung cancer and prostate cancer (PMID: 19176311; 28407749).

Protocols

Product Specific Protocols
WB protocol for OPCML antibody 17962-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols