Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue, pig brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27885-1-AP targets GRIA1 in WB, ELISA applications and shows reactivity with Human, Mouse, Pig, rat samples.
| Tested Reactivity | Human, Mouse, Pig, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag27295 Product name: Recombinant human GRIA1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 88-287 aa of BC111734 Sequence: FYERRTVNMLTSFCGALHVCFITPSFPVDTSNQFVLQLRPELQDALISIIDHYKWQKFVYIYDADRGLSVLQKVLDTAAEKNWQVTAVNILTTTEEGYRMLFQDLEKKKERLVVVDCESERLNAILGQIIKLEKNGIGYHYILANLGFMDIDLNKFKESGANVTGFQLVNYTDTIPAKIMQQWKNSDARDHTRVDWKRPK Predict reactive species |
| Full Name | glutamate receptor, ionotropic, AMPA 1 |
| Calculated Molecular Weight | 906 aa, 102 kDa |
| Observed Molecular Weight | 102 kDa |
| GenBank Accession Number | BC111734 |
| Gene Symbol | GRIA1 |
| Gene ID (NCBI) | 2890 |
| RRID | AB_3086003 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P42261 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GRIA1 antibody 27885-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
