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Tested Applications
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
30156-1-AP targets LRRC50 in IHC, IF-P, ELISA applications and shows reactivity with Human, mouse samples.
| Tested Reactivity | Human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag32822 Product name: Recombinant human LRRC50 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 522-680 aa of BC024009 Sequence: FVTELDGTRTEDLETIRLETKETCCIDDLPDLEDDDETGKSLEDQNMCFPKIEVISSLSDDSDPELDYTSLPVLENLPTDTLSNIFAVSKDTSKAARVPFTDIFKKEAKRDSEIRKQDTKSPRPLIQELSDEDPSGQPLMPPTCQRDAAPLTSTGDRDS Predict reactive species |
| Full Name | leucine rich repeat containing 50 |
| Calculated Molecular Weight | 725 aa, 80 kDa |
| GenBank Accession Number | BC024009 |
| Gene Symbol | LRRC50 |
| Gene ID (NCBI) | 123872 |
| RRID | AB_3086249 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8NEP3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LRRC50 is a cilium-specific protein required for the stability of the ciliary architecture, it plays a role in cytoplasmic preassembly of dynein arms.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for LRRC50 antibody 30156-1-AP | Download protocol |
| IHC protocol for LRRC50 antibody 30156-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
