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  • Western Blot (WB) analysis of various lysates using LACE1 Polyclonal antibody (30157-1-AP)

    WB analysis using 30157-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30157-1-AP (LACE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LACE1 Polyclonal antibody (30157-1-AP)

    IF Staining of HepG2 using 30157-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LACE1 antibody (30157-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using LACE1 Polyclonal antibody (30157-1-AP)

    IF Staining of U2OS using 30157-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using LACE1 antibody (30157-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

LACE1 Polyclonal antibody

LACE1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 30157-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

AFG1, AFG1L, AFG1-like ATPase, EC:3.6.-.-, Lactation elevated protein 1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inA549 cells, HuH-7 cells, mouse liver tissue, rat liver tissue
Positive IF/ICC detected inU2OS cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30157-1-AP targets LACE1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag32827

Product name: Recombinant human LACE1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 14-313 aa of BC018445

Sequence: LAQSPLRGRCVGCGAWAAALAPLATAPGKPFWKAYTVQTSESMTPTATSETYLKALAVCHGPLDHYDFLIKAHELKDDEHQRRVIQCLQKLHEDLKGYNIEAEGLFSKLFSRSKPPRGLYVYGDVGTGKTMVMDMFYAYVEMKRKKRVHFHGFMLDVHKRIHRLKQSLPKRKPGFMAKSYDPIAPIAEEISEEACLLCFDEFQVTDIADAMILKQLFENLFKNGVVVVATSNRPPEDLYKNGLQRANFVPFIAVLKEYCNTVQLDSGIDYRKRELPAAGKLYYLTSEADVEAVMDKLFDE

Predict reactive species
Full Name lactation elevated 1
Calculated Molecular Weight54 kDa
Observed Molecular Weight54-58 kda
GenBank Accession NumberBC018445
Gene Symbol LACE1
Gene ID (NCBI) 246269
RRIDAB_3086250
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WV93
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for LACE1 antibody 30157-1-APDownload protocol
WB protocol for LACE1 antibody 30157-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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