Tested Applications
| Positive WB detected in | Transfected with Human IL22 CHO cells, Recombinant protein |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
82766-15-RR targets IL-22 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Recombinant protein Predict reactive species |
| Full Name | interleukin 22 |
| Observed Molecular Weight | 20 kDa |
| GenBank Accession Number | BC066263 |
| Gene Symbol | IL-22 |
| Gene ID (NCBI) | 50616 |
| RRID | AB_3743428 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9GZX6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Interleukin-22 (IL-22) is a member of the IL-10 cytokine family, primarily produced by immune cells such as group 3 innate lymphoid cells (ILC3s), Th17 cells, Th22 cells, and γδ T cells (PMID: 25706098). Its unique feature lies in targeting non-immune epithelial and stromal cells, which exclusively express the heterodimeric IL-22 receptor (IL-22R1 and IL-10R2). IL-22 plays a dual role in immunity and tissue homeostasis: it promotes epithelial cell proliferation, regeneration, and barrier integrity through the upregulation of antimicrobial peptides (e.g., defensins) and mucin production, thereby enhancing defense against extracellular pathogens. However, IL-22 can also drive pathological inflammation in autoimmune and chronic inflammatory diseases (e.g., psoriasis, colitis) when dysregulated. This functional dichotomy highlights its context-dependent nature as both a protective mediator and a pathogenic effector (PMID: 20498020).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IL-22 antibody 82766-15-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
IL-22 Recombinant monoclonal antibody (250428B5), catalog number 82766-15-RR, has a total of 2 citations. These citations appear in the International Journal of Biological Macromolecules and the International Journal of Molecular Sciences. The associated research fields include gut microbiota modulation, colitis, colorectal cancer, and tryptophan metabolism-mediated signaling pathways.
| Species | Application | Title |
|---|---|---|
Int J Biol Macromol Multi-omics insights into anti-colitis benefits of the synbiotic and postbiotic derived from wheat bran arabinoxylan and Limosilactobacillus reuteri. | ||
Int J Mol Sci 20(S/R)-Ginsenoside Rh1 Alleviates AOM/DSS-Induced Colorectal Cancer: Gut-Microbiota Modulation and Tryptophan-Metabolism-Mediated AhR/PXR Activation and IDO1. |
