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  • Western Blot (WB) analysis of various lysates using CARHSP1 Recombinant antibody (83300-3-RR)

    WB analysis using 83300-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83300-3-RR (CARHSP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using CARHSP1 Recombinant antibody (83300-3-RR)

    WB analysis of HEK-293 using 83300-3-RR

    WB result of CARHSP1 antibody (83300-3-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CARHSP1 transfected HEK-293 cells.

  • Flow cytometry (FC) experiment of HepG2 cells using CARHSP1 Recombinant antibody (83300-3-RR)

    FC experiment of HepG2 using 83300-3-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CARHSP1 Recombinant antibody (83300-3-RR, Clone:230489A2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CARHSP1 Recombinant antibody

CARHSP1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83300-3-RR
Clone No.230489A2

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

CSDC1, CRHSP-24, CRHSP 24, Calcium-regulated heat-stable protein of 24 kDa, Calcium-regulated heat-stable protein 1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, HepG2 cells, HEK-293T cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83300-3-RR targets CARHSP1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CARHSP1 fusion protein Ag2265 Predict reactive species
Full Name calcium regulated heat stable protein 1, 24kDa
Calculated Molecular Weight 147 aa, 16 kDa
Observed Molecular Weight22 kDa
GenBank Accession NumberBC003366
Gene Symbol CARHSP1
Gene ID (NCBI) 23589
RRIDAB_3670970
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9Y2V2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CARHSP1(calcium-regulated heat-stable protein 1, a 24 kDa protein also known as CRHSP-24) is a ubiquitously expressed phosphoprotein that is comprised of 147 amino acids with nearly 14% proline (PMID: 21990353,30257675). CARHSP1 was discovered as a biomarker for diabetic retinopathy and as a regulator of TNF-α mRNA stability in macrophages (PMID: 21078874). CARHSP1 may be involved in oxidative stress via traffic between stress granules and processing bodies (PMID: 21177848).

Protocols

Product Specific Protocols
WB protocol for CARHSP1 antibody 83300-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols