Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, K-562 cells, Jurkat cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83509-5-RR targets FBXO38 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag35444 Product name: Recombinant human FBXO38 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 490-700 aa of XM_005268513.1 Sequence: SNQNSNNDDNNAQNNNANIHDNNHHHPDDSDEENDFRQDLQPGEQQFAADALNEMEDIVQEDGEVVAESGNNTPAHSQAIIPVDVDEEQAGPSGLQRVVKPTSITVHDSESDDEEDSLELQEVWIPKNGTRRYSEREEKTGESVQSRELSVSGKGKTPLRKRYNSHQMGQSKQFPLEESSCEKGCQVTSEQIKADMKAARDIPEKKKNKDV Predict reactive species |
| Full Name | F-box protein 38 |
| Calculated Molecular Weight | 134kDa，1188aa |
| Observed Molecular Weight | 125kDa, 140 kDa, 150kDa |
| GenBank Accession Number | XM_005268513.1 |
| Gene Symbol | FBXO38 |
| Gene ID (NCBI) | 81545 |
| RRID | AB_3671133 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q6PIJ6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
F-box-containing protein 38 (FBXO38), a member of the F-box family, was initially discovered as a co-activator of the transcription factor Krüppel-like factor 7 (KLF7) (PMID: 14729953). FBXO38 acts as a substrate-binding receptor for multi-subunit ubiquitin ligase SKP1-CUL1-FBXO38 (PMID: 35769260) . FBXO38 is an E3 ligase of PD-1 that mediates Lys48-linked poly-ubiquitination and subsequent proteasome degradation (PMID: 30487606).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for FBXO38 antibody 83509-5-RR | Download protocol |
| WB protocol for FBXO38 antibody 83509-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
