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CNOT4 Recombinant monoclonal antibody, PBS Only (83615-4-PBS)

Filter: AllWBIHCIF/ICCFC (Intra)Affinity

WB analysis using 83615-4-RR (same clone as 83615-4-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 83615-4-RR (CNOT4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83615-4-PBS in a different storage buffer formulation.
IHC staining of human ovary cancer using 83615-4-RR (same clone as 83615-4-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83615-4-RR (CNOT4 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83615-4-PBS in a different storage buffer formulation.
IF Staining of HeLa using 83615-4-RR (same clone as 83615-4-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CNOT4 antibody (83615-4-RR, Clone: 240559E1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83615-4-PBS in a different storage buffer formulation.
FC experiment of HeLa using 83615-4-RR (same clone as 83615-4-PBS)
1x10^6 HeLa cells were intracellularly stained with 0.25 ug Cnot4 Recombinant Antibody (83615-4-RR, Clone:240559E1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83615-4-PBS in a different storage buffer formulation.
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1x10^6 HeLa cells were intracellularly stained with 0.25 ug Cnot4 Recombinant Antibody (83615-4-RR, Clone:240559E1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83615-4-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 83615-4-RR
Biolayer interferometry (BLl) kinetic assays of 83615-4-RR against Human CNOT4 were performed. The affinity constant is 0.485 nM.

CNOT4 Recombinant monoclonal antibody, PBS Only

Name: CNOT4 Recombinant monoclonal antibody, PBS Only
Brand: Proteintech
SKU: 83615-4-PBS

CNOT4 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 83615-4-PBS

Clone No.240559E1

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

240559E1, CCR4 associated factor 4, CCR4-associated factor 4, CCR4-NOT transcription complex subunit 4, CLONE243

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

Print datasheet

MSDS SDS

Product Information

83615-4-PBS targets CNOT4 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag3163 Product name: Recombinant human CNOT4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 273-572 aa of BC035590 Sequence: IDKPSDSLSIGNGDNSQQISNSDTPSPPPGLSKSNPVIPISSSNHSARSPFEGAVTESQSLFSDIFRHPNPIPSGLPPFPSSPQTSSDWPTAPEPQSLFTSETIPVSSSTDWQAAFGFGSSKQPEDDLGFDPFDVTRKALADLIEKELSVQDQPSLSPTSLQNSSSHTTTAKGPGSGFLHPAAATNANSLNSTFSVLPQRFPQFQQHRAVYNSFSFPGQAARYPWMAFPRNSIMHLNHTANPTSNSNFLDLNLPPQHNTGLGGIPVAGEEEVKVSTMPLSTSSHSLQQGQQPTSLHTTVA Predict reactive species
Full Name	CCR4-NOT transcription complex, subunit 4
Calculated Molecular Weight	575 aa, 64 kDa
Observed Molecular Weight	64-85 kDa
GenBank Accession Number	BC035590
Gene Symbol	CNOT4
Gene ID (NCBI)	4850
RRID	AB_3671229
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	O95628
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

CNOT4, also known as CCR4-NOT transcription complex subunit 4 , has E3 ubiquitin ligase activity, and promotes ubiquitination and degradation of target proteins. CNOT4 is involved in activation of the JAK/STAT pathway. CNOT4 exist many isoforms and the range of its molecular weight from 48 to 84 kDa.