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  • Western Blot (WB) analysis of various lysates using CDH26 Recombinant antibody (84143-1-RR)

    WB analysis using 84143-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84143-1-RR (CDH26 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of U-2 OS using CDH26 Recombinant antibody (84143-1-RR)

    FC experiment of U-2 OS using 84143-1-RR

    1x10^6 U-2 OS were intracellularly stained with 0.25 ug CDH26 Recombinant antibody (84143-1-RR, Clone:240934F9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of HeLa cells using CDH26 Recombinant antibody (84143-1-RR)

    FC experiment of HeLa using 84143-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug CDH26 Recombinant antibody (84143-1-RR, Clone:240934F9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CDH26 Recombinant antibody

CDH26 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84143-1-RR
Clone No.240934F9

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

Cadherin-like protein VR20, Cadherin-like protein 26, 240934F9

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inA549 cells, MCF-7 cells, HEK-293 cells, T-47D cells
Positive FC (Intra) detected inU-2 OS, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84143-1-RR targets CDH26 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide Predict reactive species
Full Name cadherin-like 26
Calculated Molecular Weight 95 kDa
Observed Molecular Weight18 kDa
GenBank Accession NumberNM_021810
Gene Symbol CDH26
Gene ID (NCBI) 60437
RRIDAB_3671705
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ8IXH8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDH26, also named VR20, is a member of cadherins which are calcium-dependent cell adhesion proteins. Cadherins preferentially interact with themselves in a homophilic manner in connecting cells. Cadherins may thus contribute to the sorting of heterogeneous cell types. CDH26 produces 4 isoforms through alternative splicing, with molecular weights of 13, 18, 92, and 95 kDa, respectively.

Protocols

Product Specific Protocols
WB protocol for CDH26 antibody 84143-1-RRDownload protocol
FC protocol for CDH26 antibody 84143-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols