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  • Western Blot (WB) analysis of various lysates using Caspase 10 Recombinant antibody (85196-3-RR)

    WB analysis using 85196-3-RR

    Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 85196-3-RR (Caspase 10 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Caspase 10 Recombinant antibody (85196-3-RR)

    WB analysis using 85196-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85196-3-RR (Caspase 10 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of Jurkat cells using Caspase 10 Recombinant antibody (85196-3-RR)

    IHC staining of Jurkat using 85196-3-RR

    Immunohistochemical analysis of paraffin-embedded Jurkat cells slide using 85196-3-RR (Caspase 10 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using Caspase 10 Recombinant antibody (85196-3-RR)

    IF Staining of Jurkat using 85196-3-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using Caspase 10 antibody (85196-3-RR, Clone: 242353H6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HepG2 cells using Caspase 10 Recombinant antibody (85196-3-RR)

    FC experiment of HepG2 using 85196-3-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Caspase 10 Recombinant Antibody (85196-3-RR, Clone:242353H6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Caspase 10 Recombinant monoclonal antibody

Caspase 10 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 85196-3-RR
Clone No.242353H6

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

CASP10, Caspase10, 242353H6, Apoptotic protease Mch 4, Apoptotic protease Mch-4

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inHepG2 cells, Jurkat cells, A431 cells, K-562 cells
Positive IHC detected inJurkat cells
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inJurkat cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85196-3-RR targets Caspase 10 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

Predict reactive species
Full Name caspase 10, apoptosis-related cysteine peptidase
Calculated Molecular Weight 59 kDa
Observed Molecular Weight59 kDa
GenBank Accession NumberBC042844
Gene Symbol Caspase 10
Gene ID (NCBI) 843
Conjugate Unconjugated
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ92851
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CASP10(Caspase-10) is also named as MCH4 and belongs to the peptidase C14A family. It is recruited to the CD99 and p55 tumor necrosis factor receptor signaling complexes and promoting CD95 (APO1/Fas) apoptosis. It may also play a critical role in PTK7-knockdown-induced apoptosis, downstream of mitochondria(PMID:21103379). This protein can be Cleavage by granzyme B and autocatalytic activity generate two active subunits. Defects in CASP10 are the cause of autoimmune lymphoproliferative syndrome type 2A (ALPS2A)(PMID:16446975), familial non-Hodgkin lymphoma (NHL)(PMID:12010812) and gastric cancer (GASC)(PMID:11973654). The full length has a propeptide with 219 amino acid and 6 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
FC protocol for Caspase 10 antibody 85196-3-RRDownload protocol
IF protocol for Caspase 10 antibody 85196-3-RRDownload protocol
IHC protocol for Caspase 10 antibody 85196-3-RRDownload protocol
WB protocol for Caspase 10 antibody 85196-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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