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  • Western Blot (WB) analysis of Daudi cells using MARCH1 Recombinant antibody (85308-2-RR)

    WB analysis of Daudi using 85308-2-RR

    Daudi cells were subjected to SDS PAGE followed by western blot with 85308-2-RR (MARCH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using MARCH1 Recombinant antibody (85308-2-RR)

    WB analysis using 85308-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85308-2-RR (MARCH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MARCH1 Recombinant antibody (85308-2-RR)

    IHC staining of mouse brain using 85308-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85308-2-RR (MARCH1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 85308-2-RR

    Affinity and Kinetic Characterization of 85308-2-RR

    Biolayer interferometry (BLl) kinetic assays of 85308-2-RR against Human MARCH1 were performed. The affinity constant is 29.7 nM.

MARCH1 Recombinant antibody

MARCH1 Recombinant Antibody for WB, IHC, ELISA

Cat No. 85308-2-RR
Clone No.242143G10

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

MARCHF1, E3 ubiquitin-protein ligase MARCHF1, EC:2.3.2.27, MARCH I, MARCH-I

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inmouse brain tissue, Daudi cells, rat brain tissue, human skeletal muscle tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85308-2-RR targets MARCH1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MARCH1 fusion protein Ag20231 Predict reactive species
Full Name membrane-associated ring finger (C3HC4) 1
Calculated Molecular Weight 289 aa, 32 kDa
Observed Molecular Weight31-35 kDa
GenBank Accession NumberBC153124
Gene Symbol MARCHF1
Gene ID (NCBI) 55016
Conjugate Unconjugated
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ8TCQ1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MARCH1 is mainly found in secondary lymphoid organs, more specifically in the endocytic pathway of dendritic cells (DCs) and B cells (11-15). MARCH1 reduces the half-life of peptide/MHC II complexes by causing their redistribution from recycling endosomes to lysosomes. MARCH1 homodimerizes and also forms heterodimers with others family members (PMID: 22508929).

Protocols

Product Specific Protocols
WB protocol for MARCH1 antibody 85308-2-RRDownload protocol
IHC protocol for MARCH1 antibody 85308-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols