Various lysates were subjected to SDS PAGE followed by western blot with 85389-1-RR (Cas9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 85389-1-RR (Cas9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Affinity and Kinetic Characterization of 85389-1-RR
Biolayer interferometry (BLl) kinetic assays of 85389-1-RR against Human Cas9 were performed. The affinity constant is 3.18 nM.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
Cas9 (CRISPR associated protein 9) is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) adaptive immunity system in Streptococcus pyogenes. This antibody detects the sp Cas9.
Various lysates were subjected to SDS PAGE followed by western blot with 85389-1-RR (Cas9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
AFFINITY Figures
Affinity and Kinetic Characterization of 85389-1-RR
Biolayer interferometry (BLl) kinetic assays of 85389-1-RR against Human Cas9 were performed. The affinity constant is 3.18 nM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
Cas9 Recombinant antibody
Catalog Number
85389-1-RR
Citations
-
Dilutions
WB : 1:5000-1:50000
Applications
WB, ELISA
Reactivity
n/a
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
