Tested Applications
| Positive WB detected in | mouse small intestine tissue, mouse liver tissue, rat liver tissue, mouse colon tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85671-2-RR targets IL-15RA in WB, ELISA applications and shows reactivity with mouse, rat samples.
| Tested Reactivity | mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | IL-15RA fusion protein Eg2687 Predict reactive species |
| Full Name | interleukin 15 receptor, alpha chain |
| Calculated Molecular Weight | 28 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | NM_008358.2 |
| Gene Symbol | Il15ra |
| Gene ID (NCBI) | 16169 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q60819-1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Interleukin-15 (IL-15) is a pleiotropic cytokine that plays an important role in both innate and adaptive immunity. IL-15 is mainly produced by activated monocytes, macrophages and dendritic cells, and is structurally similar to IL-2 (PMID: 8178155; 12401478). These two cytokines share the same IL-2/15Rβ and common γ-chain receptor subunits. In addition, IL-2 and IL-15 have their own private α-chain receptor subunit, IL-2Rα and IL-15Rα, respectively (PMID: 7641685). The IL-15Rα chain is expressed by many cell types including monocytes, DCs, NK, T cells and fibroblasts. Several isoforms of IL-15Rα exist and are generated either by alternative splicing or by proteolytic cleavage (PMID: 10480910; 15265897). The calculated molecular weight of full-length IL-15Rα is 28 kDa, IL-15Rα can be glycosylated, and larger apparent molecular weights of 55-65 kDa have been reported by some researches (PMID: 10480910; 15976182; 15671076; 17912462).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IL-15RA antibody 85671-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
