Mouse IL-15RA Recombinant antibody, PBS Only (Detector)

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  WB analysis using 85671-2-RR (same clone as 85671-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85671-2-RR (Il15ra antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85671-2-PBS in a different storage buffer formulation.

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  WB analysis using 85671-2-RR (same clone as 85671-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85671-2-RR (Il15ra antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85671-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP02016-3

  Sandwich ELISA standard curve of MP02016-3, Mouse IL-15RA Recombinant Matched Antibody Pair - PBS only. 85671-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2687. 85671-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

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  Sample test of MP02016-3

  The mean IL-15RA concentration was determined to be 1,332.06 ng/mL in mouse liver tissue extract based on a 3.7 mg/mL extract load.

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  Cytometric bead array standard curve of MP02016-1

  Cytometric bead array standard curve of MP02016-1, MOUSE IL-15RA Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 98406-2-PBS. Detection antibody: 85671-2-PBS. Standard: Eg2687. Range: 0.781-100 ng/mL.

IL-15RA Uni-rAb^TM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 85671-2-PBS

Clone No.242618H6

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

CD215, IL-15 receptor subunit alpha, Il15ra, IL-15R-alpha, Interleukin-15 receptor subunit alpha

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Product Information

85671-2-PBS targets IL-15RA as part of a matched antibody pair:

MP02016-1: 98406-2-PBS capture and 85671-2-PBS detection (validated in Cytometric bead array)

MP02016-3: 85671-4-PBS capture and 85671-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: IL-15RA fusion protein Eg2687
• Full Name: interleukin 15 receptor, alpha chain
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: NM_008358.2
• Gene Symbol: Il15ra
• Gene ID (NCBI): 16169
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q60819-1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Interleukin-15 (IL-15) is a pleiotropic cytokine that plays an important role in both innate and adaptive immunity. IL-15 is mainly produced by activated monocytes, macrophages and dendritic cells, and is structurally similar to IL-2 (PMID: 8178155; 12401478). These two cytokines share the same IL-2/15Rβ and common γ-chain receptor subunits. In addition, IL-2 and IL-15 have their own private α-chain receptor subunit, IL-2Rα and IL-15Rα, respectively (PMID: 7641685). The IL-15Rα chain is expressed by many cell types including monocytes, DCs, NK, T cells and fibroblasts. Several isoforms of IL-15Rα exist and are generated either by alternative splicing or by proteolytic cleavage (PMID: 10480910; 15265897). The calculated molecular weight of full-length IL-15Rα is 28 kDa, IL-15Rα can be glycosylated, and larger apparent molecular weights of 55-65 kDa have been reported by some researches (PMID: 10480910; 15976182; 15671076; 17912462).