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  • Western Blot (WB) analysis of various lysates using CA4 Recombinant antibody (85706-1-RR)

    WB analysis using 85706-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85706-1-RR (CA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using CA4 Recombinant antibody (85706-1-RR)

    IHC staining of mouse lung using 85706-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 85706-1-RR (CA4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 85706-1-RR

    Affinity and Kinetic Characterization of 85706-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85706-1-RR against Human CA4 were performed. The affinity constant is 9.62 nM.

CA4 Recombinant antibody

CA4 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 85706-1-RR
Clone No.242975A3

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

CA IV, CAIV, CA-IV, Car4, Carbonate dehydratase IV

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inmouse lung tissue, rat lung tissue
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85706-1-RR targets CA4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CA4 fusion protein Ag4960 Predict reactive species
Full Name carbonic anhydrase IV
Calculated Molecular Weight 35 kDa
Observed Molecular Weight35 kDa
GenBank Accession NumberBC057792
Gene Symbol CA4
Gene ID (NCBI) 762
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP22748
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CA4(Carbonic anhydrase 4) is a glycosylphosphatidyl-inositol-anchored membrane isozyme expressed on the luminal surfaces of pulmonary, chorionic (and certain other) capillaries and of proximal renal tubules. It plays a critical role by maintaining the pH in the outer retina, which is important for the normal function of photoreceptors. This protein belongs to the alpha-carbonic anhydrase family.

Protocols

Product Specific Protocols
WB protocol for CA4 antibody 85706-1-RRDownload protocol
IHC protocol for CA4 antibody 85706-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols