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  • Western Blot (WB) analysis of various lysates using IRF2BP1 Recombinant antibody (86048-1-RR)

    WB analysis using 86048-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86048-1-RR (IRF2BP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using IRF2BP1 Recombinant antibody (86048-1-RR)

    WB analysis using 86048-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86048-1-RR (IRF2BP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using IRF2BP1 Recombinant antibody (86048-1-RR)

    IF Staining of U2OS using 86048-1-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using IRF2BP1 antibody (86048-1-RR, Clone: 250630H5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

IRF2BP1 Recombinant antibody

IRF2BP1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86048-1-RR
Clone No.250630H5

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

EC:2.3.2.27, Interferon regulatory factor 2-binding protein 1, IRF 2 binding protein 1, IRF 2BP1, IRF-2-binding protein 1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue, HEK-293 cells, Jurkar cells, rat brain tissue
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86048-1-RR targets IRF2BP1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IRF2BP1 fusion protein Ag4596 Predict reactive species
Full Name interferon regulatory factor 2 binding protein 1
Calculated Molecular Weight 584 aa, 62 kDa
Observed Molecular Weight65-70 kDa
GenBank Accession NumberBC038222
Gene Symbol IRF2BP1
Gene ID (NCBI) 26145
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8IU81
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IRF2BP1 inhibits IRF2-mediated transcriptional activation by interacting with IRF2. In epidermal growth factor receptor (EGFR) signaling, transient deSUMOylation of IRF2BP1 is essential for the proper expression of immediate early genes such as DUSP1 and ATF3. IRF2BP1 is also involved in the TGF-β signaling pathway by controlling the accumulation of cPML in the cytoplasm, which in turn affects the phosphorylation of Smad2 and the assembly of the Smad2-receptor complex.

Protocols

Product Specific Protocols
WB protocol for IRF2BP1 antibody 86048-1-RRDownload protocol
IF protocol for IRF2BP1 antibody 86048-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols