Tested Applications
Positive WB detected in | HeLa cells, mouse brain tissue, HEK-293 cells, Jurkar cells, rat brain tissue |
Positive IF/ICC detected in | U2OS cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
86048-1-RR targets IRF2BP1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | IRF2BP1 fusion protein Ag4596 Predict reactive species |
Full Name | interferon regulatory factor 2 binding protein 1 |
Calculated Molecular Weight | 584 aa, 62 kDa |
Observed Molecular Weight | 65-70 kDa |
GenBank Accession Number | BC038222 |
Gene Symbol | IRF2BP1 |
Gene ID (NCBI) | 26145 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q8IU81 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IRF2BP1 inhibits IRF2-mediated transcriptional activation by interacting with IRF2. In epidermal growth factor receptor (EGFR) signaling, transient deSUMOylation of IRF2BP1 is essential for the proper expression of immediate early genes such as DUSP1 and ATF3. IRF2BP1 is also involved in the TGF-β signaling pathway by controlling the accumulation of cPML in the cytoplasm, which in turn affects the phosphorylation of Smad2 and the assembly of the Smad2-receptor complex.
Protocols
Product Specific Protocols | |
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WB protocol for IRF2BP1 antibody 86048-1-RR | Download protocol |
IF protocol for IRF2BP1 antibody 86048-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |