IRF2BP1 Recombinant antibody, PBS Only

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  WB analysis using 86048-1-RR (same clone as 86048-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86048-1-RR (IRF2BP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86048-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 86048-1-RR (same clone as 86048-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86048-1-RR (IRF2BP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86048-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U2OS using 86048-1-RR (same clone as 86048-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using IRF2BP1 antibody (86048-1-RR, Clone: 250630H5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86048-1-PBS in a different storage buffer formulation.

IRF2BP1 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 86048-1-PBS

Clone No.250630H5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

EC:2.3.2.27, Interferon regulatory factor 2-binding protein 1, IRF 2 binding protein 1, IRF 2BP1, IRF-2-binding protein 1

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Product Information

86048-1-PBS targets IRF2BP1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: IRF2BP1 fusion protein Ag4596
• Full Name: interferon regulatory factor 2 binding protein 1
• Calculated Molecular Weight: 584 aa, 62 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC038222
• Gene Symbol: IRF2BP1
• Gene ID (NCBI): 26145
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8IU81
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

IRF2BP1 inhibits IRF2-mediated transcriptional activation by interacting with IRF2. In epidermal growth factor receptor (EGFR) signaling, transient deSUMOylation of IRF2BP1 is essential for the proper expression of immediate early genes such as DUSP1 and ATF3. IRF2BP1 is also involved in the TGF-β signaling pathway by controlling the accumulation of cPML in the cytoplasm, which in turn affects the phosphorylation of Smad2 and the assembly of the Smad2-receptor complex.