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  • Western Blot (WB) analysis of various lysates using HPRT Recombinant antibody (86049-1-RR)

    WB analysis using 86049-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86049-1-RR (HPRT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HPRT Recombinant antibody (86049-1-RR)

    IHC staining of human breast cancer using 86049-1-RR

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 86049-1-RR (HPRT antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using HPRT Recombinant antibody (86049-1-RR)

    IHC staining of human colon cancer using 86049-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 86049-1-RR (HPRT antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using HPRT Recombinant antibody (86049-1-RR)

    IHC staining of human colon cancer using 86049-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 86049-1-RR (HPRT antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HPRT Recombinant antibody (86049-1-RR)

    IHC staining of human breast cancer using 86049-1-RR

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 86049-1-RR (HPRT antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

HPRT Recombinant antibody

HPRT Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 86049-1-RR
Clone No.250656C2

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

HPRT1, EC:2.4.2.8, HGPRT, HGPRTase, Hypoxanthine-guanine phosphoribosyltransferase

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, Y79 cells, NIH/3T3 cells, HSC-T6 cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86049-1-RR targets HPRT in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HPRT fusion protein Ag7044 Predict reactive species
Full Name hypoxanthine phosphoribosyltransferase 1
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 24-28 kDa
GenBank Accession NumberBC000578
Gene Symbol HPRT1
Gene ID (NCBI) 3251
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP00492
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HPRT, also named HPRT1 and HGPRT, plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutation of HPRT is associated with Lesch-Nyhan syndrome (LNS) which is an X-linked inherited neurogenetic disorder of purine metabolism. It has been reported that HPRT1 also plays an important role in HPRT-related gout. Sp2/0 was reported in the literature as an HPRT1-deficient cell line. (PMID:20544509, 28045594, 27379977)

Protocols

Product Specific Protocols
WB protocol for HPRT antibody 86049-1-RRDownload protocol
IHC protocol for HPRT antibody 86049-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols