Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue, mouse cerebellum tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86905-1-RR targets CGREF1 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag35664 Product name: Recombinant human CGREF1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 24-301 aa of BC034764 Sequence: GVTRPDSEVQHQLLPNPFQPGQEQLGLLQSYLKGLGRTEVQLEHLSREQVLLYLFALHDYDQSGQLDGLELLSMLTAALAPGAANSPTTNPVILIVDKVLETQDLNGDGLMTPAELINFPGVALRHVEPGEPLAPSPQEPQAVGRQSLLAKSPLRQETQEAPGPREEAKGQVEARRESLDPVQEPGGQAEADGDVPGPRGEAEGQAEAKGDAPGPRGEAGGQAEAEGDAPGPRGEAGGQAEARENGEEAKELPGETLESKNTQNDFEVHIVQVENDEI Predict reactive species |
| Full Name | cell growth regulator with EF-hand domain 1 |
| Calculated Molecular Weight | 301 aa, 32 kDa |
| Observed Molecular Weight | 20-22 kDa |
| GenBank Accession Number | BC034764 |
| Gene Symbol | CGREF1 |
| Gene ID (NCBI) | 10669 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q99674 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CGREF1, also known as CGR11, is predicted to enable calcium ion binding activity and to be involved in negative regulation of cell population proliferation. Human GREF1 protein has 4 clustered 17-amino acid repeats within the C terminus, suggesting a structure resembling 4 alpha-helical domains interrupted by distinct turns. Northern blot analysis of rat tissues demonstrated expression of CGR11 predominantly in the whole brain and kidney, with limited expression in heart, lung, liver, and skeletal muscle and no expression in spleen and testis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CGREF1 antibody 86905-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
