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Tested Applications
| Positive WB detected in | HEK-293 cells, Jurkat cells, HT-1080 cells, A375 cells, HT-29 cells, HCT 116 cells, fetal human brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87147-1-RR targets MUM1 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag3371 Product name: Recombinant human MUM1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-152 aa of BC008098 Sequence: MLPDRSRAARDRANQKLVEYIVKAKGAESHLRAILKSRKPSRWLQTFLSSSQYVTCVETYLEDEGQLDLVVKYLQGVYQEVGAKVLQRTNGDRIRFILDVLLPEAIICAISAVDEVDYKTAEEKYIKGPSLSYREKEIFDNQLLEERNRRRR Predict reactive species |
| Full Name | melanoma associated antigen (mutated) 1 |
| Calculated Molecular Weight | 18 kDa, 79 kDa |
| Observed Molecular Weight | 80 kDa |
| GenBank Accession Number | BC008098 |
| Gene Symbol | MUM1 |
| Gene ID (NCBI) | 84939 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q2TAK8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MUM1, also named as EXPAND1, is an architectural component of the chromatin, which in response to DNA damage serves as an accessory factor to promote cell survival. It is a 53BP1-associated DNA damage-responsive factor. MUM1/EXPAND1 is different to another protein IRF4/MUM1.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MUM1 antibody 87147-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
