Tested Applications
| Positive WB detected in | HeLa cells, HeLa, HepG2, HEK-293, L02, K-562, NIH-3T3, HSC-T6 cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87234-1-RR targets PDIA6 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg5454 Product name: Recombinant Human PDIA6 protein (rFc Tag) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 20-440 aa of NM_005742.3 Sequence: LYSSSDDVIELTPSNFNREVIQSDSLWLVEFYAPWCGHCQRLTPEWKKAATALKDVVKVGAVDADKHHSLGGQYGVQGFPTIKIFGSNKNRPEDYQGGRTGEAIVDAALSALRQLVKDRLGGRSGGYSSGKQGRSDSSSKKDVIELTDDSFDKNVLDSEDVWMVEFYAPWCGHCKNLEPEWAAAASEVKEQTKGKVKLAAVDATVNQVLASRYGIRGFPTIKIFQKGESPVDYDGGRTRSDIVSRALDLFSDNAPPPELLEIINEDIAKRTCEEHQLCVVAVLPHILDTGAAGRNSYLEVLLKLADKYKKKMWGWLWTEAGAQSELETALGIGGFGYPAMAAINARKMKFALLKGSFSEQGINEFLRELSFGRGSTAPVGGGAFPTIVEREPWDGRDGELPVEDDIDLSDVELDDLGKDEL Predict reactive species |
| Full Name | protein disulfide isomerase family A, member 6 |
| Calculated Molecular Weight | 48 kDa |
| Observed Molecular Weight | 54 kDa |
| GenBank Accession Number | NM_005742.3 |
| Gene Symbol | PDIA6 |
| Gene ID (NCBI) | 10130 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q15084-1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PDIA6(Protein disulfide-isomerase A6) is also named as ERP5, P5, TXNDC7 and belongs to the protein disulfide isomerase family.It is one of the endoplasmic reticulum (ER) resident proteins that catalyze formation, reduction, and isomerization of disulfide bonds in proteins and are thought to play a role in folding of disulfide-bonded proteins(PMID:7590364).It has 2 isoforms produced by alternative splicing.This antibody is specific to PDIA6.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PDIA6 antibody 87234-1-RR | Download protocol |
| WB protocol for PDIA6 antibody 87234-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
