Tested Applications
| Positive WB detected in | HT-1080 cells, SKOV-3 cells, SiHa cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87490-1-RR targets DYNC1LI2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag13452 Product name: Recombinant human DYNC1LI2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-492 aa of BC025959 Sequence: MAPVGVEKKLLLGPNGPAVAAAGDLTSEEEEGQSLWSSILSEVSTRARSKLPSGKNILVFGEDGSGKTTLMTKLQGAEHGKKGRGLEYLYLSVHDEDRDDHTRCNVWILDGDLYHKGLLKFAVSAESLPETLVIFVADMSRPWTVMESLQKWASVLREHIDKMKIPPEKMRELERKFVKDFQDYMEPEEGCQGSPQRRGPLTSGSDEENVALPLGDNVLTHNLGIPVLVVCTKCDAVSVLEKEHDYRDEHLDFIQSHLRRFCLQYGAALIYTSVKEEKNLDLLYKYIVHKTYGFHFTTPALVVEKDAVFIPAGWDNEKKIAILHENFTTVKPEDAYEDFIVKPPVRKLVHDKELAAEDEQVFLMKQQSLLAKQPATPTRASESPARGPSGSPRTQGRGGPASVPSSSPGTSVKKPDPNIKNNAASEGVLASFFNSLLSKKTGSPGSPGAGGVQSTAKNSGQKTVLSNVQEELDRMTRKPDSMVTNSSTENEA Predict reactive species |
| Full Name | dynein, cytoplasmic 1, light intermediate chain 2 |
| Calculated Molecular Weight | 492 aa, 54 kDa |
| Observed Molecular Weight | 48-55 kDa |
| GenBank Accession Number | BC025959 |
| Gene Symbol | DYNC1LI2 |
| Gene ID (NCBI) | 1783 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O43237 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Cytoplasmic dynein is a microtubule-associated motor protein. DYNC1LI2 (Cytoplasmic dynein 1 light intermediate chain 2), also known as LIC2, belongs to the dynein light intermediate chain family. DYNC1LI2 acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. It may play a role in binding dynein to membranous organelles or chromosomes. Northern blot analysis of rat tissues detected high expression of DYNC1LI2 in skeletal muscle, testis, heart, lung, and brain, and lower expression in liver, kidney, and spleen (PMID: 7738094).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DYNC1LI2 antibody 87490-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
