DYNC1LI2 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 87490-1-RR (same clone as 87490-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 87490-1-RR (DYNC1LI2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87490-1-PBS in a different storage buffer formulation.

• 

  Affinity of 87490-1-RR

  Biolayer interferometry (BLl) kinetic assays of 87490-1-RR against Human DYNC1LI2 were performed. The affinity constant is 1.46 nM.

DYNC1LI2 Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 87490-1-PBS

Clone No.252844F3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

DNCLI2, LIC 2, LIC2, LIC-2, LIC53/55

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Product Information

87490-1-PBS targets DYNC1LI2 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag13452

  Product name: Recombinant human DYNC1LI2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-492 aa of BC025959

  Sequence: MAPVGVEKKLLLGPNGPAVAAAGDLTSEEEEGQSLWSSILSEVSTRARSKLPSGKNILVFGEDGSGKTTLMTKLQGAEHGKKGRGLEYLYLSVHDEDRDDHTRCNVWILDGDLYHKGLLKFAVSAESLPETLVIFVADMSRPWTVMESLQKWASVLREHIDKMKIPPEKMRELERKFVKDFQDYMEPEEGCQGSPQRRGPLTSGSDEENVALPLGDNVLTHNLGIPVLVVCTKCDAVSVLEKEHDYRDEHLDFIQSHLRRFCLQYGAALIYTSVKEEKNLDLLYKYIVHKTYGFHFTTPALVVEKDAVFIPAGWDNEKKIAILHENFTTVKPEDAYEDFIVKPPVRKLVHDKELAAEDEQVFLMKQQSLLAKQPATPTRASESPARGPSGSPRTQGRGGPASVPSSSPGTSVKKPDPNIKNNAASEGVLASFFNSLLSKKTGSPGSPGAGGVQSTAKNSGQKTVLSNVQEELDRMTRKPDSMVTNSSTENEA

• Full Name: dynein, cytoplasmic 1, light intermediate chain 2
• Calculated Molecular Weight: 492 aa, 54 kDa
• Observed Molecular Weight: 48-55 kDa
• GenBank Accession Number: BC025959
• Gene Symbol: DYNC1LI2
• Gene ID (NCBI): 1783
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O43237
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Cytoplasmic dynein is a microtubule-associated motor protein. DYNC1LI2 (Cytoplasmic dynein 1 light intermediate chain 2), also known as LIC2, belongs to the dynein light intermediate chain family. DYNC1LI2 acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. It may play a role in binding dynein to membranous organelles or chromosomes. Northern blot analysis of rat tissues detected high expression of DYNC1LI2 in skeletal muscle, testis, heart, lung, and brain, and lower expression in liver, kidney, and spleen (PMID: 7738094).