Tested Applications
| Positive WB detected in | mouse spinal cord tissue, rat spinal cord tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87583-1-RR targets ECEL1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag5460 Product name: Recombinant human ECEL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 425-775 aa of BC050453 Sequence: AQEMEGSDKPQELARVCLGQANRHFGMALGALFVHEHFSAASKAKVQQLVEDIKYILGQRLEELDWMDAETRAAARAKLQYMMVMVGYPDFLLKPDAVDKEYEFEVHEKTYFKNILNSIRFSIQLSVKKIRQEVDKSTWLLPPQALNAYYLPNKNQMVFPAGILQPTLYDPDFPQSLNYGGIGTIIGHELTHGYDDWGGQYDRSGNLLHWWTEASYSRFLRKAECIVRLYDNFTVYNQRVNGKHTLGENIADMGGLKLAYHAYQKWVREHGPEHPLPRLKYTHDQLFFIAFAQNWCIKRRSQSIYLQVLTDKHAPEHYRVLGSVSQFEEFGRAFHCPKDSPMNPAHKCSVW Predict reactive species |
| Full Name | endothelin converting enzyme-like 1 |
| Calculated Molecular Weight | 88 kDa |
| Observed Molecular Weight | 95 kDa |
| GenBank Accession Number | BC050453 |
| Gene Symbol | ECEL1 |
| Gene ID (NCBI) | 9427 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O95672 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The M13 family has seven members, including ECEL1 (endothelin-converting enzyme-like 1), one of the newest members. ECEL1, also termed DINE in rodents, it is expressed predominantly in the central nervous system. It has been proposed that the enzyme has a role in the nervous regulation of the respiratory system(PMID:14992683).It may contribute to the degradation of peptide hormones and be involved in the inactivation of neuronal peptides.It has 2 isoforms produced by alternative splicing and 3 glycosylation sites.It can be detected a band of 95kDa in CHO cells by western blot because of the heavily glycosylated(PMID:10961933).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ECEL1 antibody 87583-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
