The mean USF2 concentration was determined to be 103.2 ng/mL in Romas cell extract based on a 1.5 mg/mL extract load, 44.9 ng/mL in PC-3 cell extract based on a 1.5 mg/mL extract load and 59.0 ng/mL in HaCaT cell extract based on a 1.4 mg/mL extract load.

Sandwich ELISA standard curve of MP03136-2, Human USF2 Recombinant Matched Antibody Pair - PBS only. 87636-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag9866. 87636-5-PBS was HRP conjugated as the detection antibody. Range: 97.7-6250 pg/mL.

The mean USF2 concentration was determined to be 30.4 ng/mL in Ramos cell extract based on a 1.2 mg/mL extract load, 25.3 ng/mL in Raji cell extract based on a 1.2 mg/mL extract load, 7.8 ng/mL in PC-3 cell extract based on a 1.2 mg/mL extract load.

Cytometric bead array standard curve of MP03136-1, USF2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 87636-2-PBS. Detection antibody: 87636-3-PBS. Standard: Ag9866. Range: 0.156-20 ng/mL.