Tested Applications
| Positive WB detected in | bEnd.3 cells, mouse lung tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87684-1-RR targets ESAM in WB, ELISA applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg6352 Product name: Recombinant mouse ESAM protein Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 30-251 aa of NM_027102.3 Sequence: QMELHVPPGLNKLEAVEGEEVVLPAWYTMAREESWSHPREVPILIWFLEQEGKEPNQVLSYINGVMTNKPGTALVHSISSRNVSLRLGALQEGDSGTYRCSVNVQNDEGKSIGHSIKSIELKVLVPPAPPSCSLQGVPYVGTNVTLNCKSPRSKPTAQYQWERLAPSSQVFFGPALDAVRGSLKLTNLSIAMSGVYVCKAQNRVGFAKCNVTLDVMTGSKAA Predict reactive species |
| Full Name | endothelial cell-specific adhesion molecule |
| Calculated Molecular Weight | 42 kDa |
| Observed Molecular Weight | 42-60 kDa |
| GenBank Accession Number | NM_027102.3 |
| Gene Symbol | Esam |
| Gene ID (NCBI) | 69524 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q925F2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ESAM, a 55 kDa type I transmembrane glycoprotein, possesses two extracellular Ig domains and a long cytoplasmic tail in which a PDZ domain is located (PMID: 22130718). The ~60 kDa band is glycosylated ESAM, and the lower band is deglycosylated ESAM.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ESAM antibody 87684-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
