Mouse ESAM Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 87684-1-RR (same clone as 87684-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 87684-1-RR (ESAM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87684-1-PBS in a different storage buffer formulation.

ESAM Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 87684-1-PBS

Clone No.253062C8

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, Indirect ELISA

Synonyms

Endothelial cell-selective adhesion molecule, Esam1

---

Product Information

87684-1-PBS targets ESAM in WB, Indirect ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg6352

  Product name: Recombinant mouse ESAM protein

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 30-251 aa of NM_027102.3

  Sequence: QMELHVPPGLNKLEAVEGEEVVLPAWYTMAREESWSHPREVPILIWFLEQEGKEPNQVLSYINGVMTNKPGTALVHSISSRNVSLRLGALQEGDSGTYRCSVNVQNDEGKSIGHSIKSIELKVLVPPAPPSCSLQGVPYVGTNVTLNCKSPRSKPTAQYQWERLAPSSQVFFGPALDAVRGSLKLTNLSIAMSGVYVCKAQNRVGFAKCNVTLDVMTGSKAA

• Full Name: endothelial cell-specific adhesion molecule
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 42-60 kDa
• GenBank Accession Number: NM_027102.3
• Gene Symbol: Esam
• Gene ID (NCBI): 69524
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q925F2
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ESAM, a 55 kDa type I transmembrane glycoprotein, possesses two extracellular Ig domains and a long cytoplasmic tail in which a PDZ domain is located (PMID: 22130718). The ~60 kDa band is glycosylated ESAM, and the lower band is deglycosylated ESAM.