Recombinant Human TNFSF13/APRIL protein (rFc Tag)
Species
Human
Purity
>90 %, SDS-PAGE
Tag
rFc Tag
Activity
not tested
Cat no : Eg3664
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <0.1 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human TNFSF13 protein Ala105-Leu250 (Accession# O75888-1) with a rabbit IgG Fc tag at the N-terminus. |
| GeneID | 8741 |
| Accession | O75888-1 |
| PredictedSize | 43.5 kDa |
| SDS-PAGE | 44-55 kDa, reducing (R) conditions |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
TNFSF13, also known as A Proliferation-Inducing Ligand (APRIL), is a member of the tumor necrosis factor (TNF) superfamily that plays significant roles in immune homeostasis, cell proliferation, and apoptosis of certain tumor cell lines. TNFSF13 is secreted from myeloid cells and is best characterized for its effects on immune cells. It plays critical roles in T cell immunity, promoting the IL-17 response and inhibiting the Th2 response, which is important in the pathogenesis of autoimmune diseases. TNFSF13 interacts with two receptors: B cell maturation antigen (BCMA) and transmembrane activator and calcium modulator and cyclophilin ligand interactor (TACI). These interactions have been shown to regulate B cell activity and can induce tumor cell invasion and immunosuppression.
References:
1. Hui-Yu Lin. et al. (2020). J Mol Med (Berl). 98(9):1255-1267. 2. Rui Chen. et al. (2021). Front Immunol. 12:713757.