SARS-CoV-2 N protein ELISA Kit

Sensitivity

38 pg/mL

Range

375-6000 pg/mL

Cat no : KE30007

Synonyms

N protein, nuclepcapsid phosphoprotein, SARS-CoV-2 N protein



Product Information

KE30007 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The SARS-CoV-2 N protein ELISA kit is to be used to detect and quantify protein levels of endogenous SARS-CoV-2 N protein. The assay recognizes human SARS-CoV-2 N protein. An antibody specific for SARS-CoV-2 N protein has been pre-coated onto the microwells. The SARS-CoV-2 N protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for SARS-CoV-2 N protein is added to detect the captured SARS-CoV-2 N protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name SARS-CoV-2 N protein ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma
Assay type Sandwich
Sensitivity 38 pg/mL
Range 375-6000 pg/mL
Reacted Species
Tested applications Sandwich ELISA

Recovery

Sample TypeAverageRange
Human plasma116%95%-127%

IntraAssay

Samplenmean ( pg/mL)SDCV%
1203011.9119.34.0
220693.738.55.5
320327.122.56.9

InterAssay

Samplenmean ( pg/mL)SDCV%
1242936.376.72.6
224682.930.14.4
324326.622.97.0

Background Information

Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.

Properties

Storage Instructions All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.
Synonyms N protein, nuclepcapsid phosphoprotein, SARS-CoV-2 N protein