ALADIN Polyclonal antibody

ALADIN Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, monkey

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15127-1-AP

Synonyms

AAA, AAAS, AAASb, ADRACALA, ADRACALIN, ALADIN, GL003



Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inCOS-7 cells, Hela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

15127-1-AP targets ALADIN in WB, IHC, IF, ELISA applications and shows reactivity with human, monkey samples.

Tested Reactivity human, monkey
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ALADIN fusion protein Ag7211
Full Name achalasia, adrenocortical insufficiency, alacrimia (Allgrove, triple-A)
Calculated molecular weight 60 kDa
Observed molecular weight 56-60 kDa
GenBank accession numberBC000659
Gene symbol AAAS
Gene ID (NCBI) 8086
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The AAAS gene product is the 60 kDa nuclear pore complex (NPC) protein alacrima-achalasia-adrenal insufficiency neurological disorder (ALADIN). AAAS mRNA and the ALADIN protein are ubiquitously expressed with predominance in the adrenal and central nervous system structures in human and rat. ALADIN, a Tryptophan-Aspartic acid (WD) repeat containing protein, was the first nuclear pore complex protein to be associated with hereditary neurodegenerative disease and the only nuclear pore complex protein to be associated with hereditary adrenal disease. ALADIN's precise function at the nuclear pore complex is unknown. Most naturally occurring AAAS mutations result in mislocalization of the abnormal ALADIN protein (mainly into the cytoplasm), implying that correct NPC targeting is vital for its function. (PMID: 23825130)

Protocols

Product Specific Protocols
WB protocol for ALADIN antibody 15127-1-APDownload protocol
IHC protocol for ALADIN antibody 15127-1-APDownload protocol
IF protocol for ALADIN antibody 15127-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Mol Biol Cell

The Nucleoporin ALADIN Regulates Aurora A Localization to Ensure Robust Mitotic Spindle Formation.

Authors - Sara Carvalhal