MRP3/ABCC3 Polyclonal antibody

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  WB analysis using 25358-1-AP

  37°C incubated HeLa cells were subjected to SDS PAGE followed by western blot with 25358-1-AP (MRP3/ABCC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 25358-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25358-1-AP (MRP3/ABCC3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 25358-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25358-1-AP (MRP3/ABCC3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 25358-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25358-1-AP (MRP3/ABCC3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MRP3/ABCC3 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 25358-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Synonyms

ABCC3, MRP3, ABC31, ATP-binding cassette sub-family C member 3, EC:7.6.2.-

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Tested Applications

• Positive WB detected in: 37°C incubated HeLa cells
• Positive IHC detected in: human colon tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

25358-1-AP targets MRP3/ABCC3 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MRP3/ABCC3 fusion protein Ag19786
• Full Name: ATP-binding cassette, sub-family C (CFTR/MRP), member 3
• Calculated Molecular Weight: 1527 aa, 169 kDa
• Observed Molecular Weight: 170-180 kDa
• GenBank Accession Number: BC137348
• Gene Symbol: MRP3
• Gene ID (NCBI): 8714
• RRID: AB_3085789
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O15438
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MRP3 also known as ABCC3, belongs to ATP-binding cassette (ABC) transporter protein superfamily. MRP3 hydrolyzes ATP to enable active transport of various substrates including many drugs, toxicants, and endogenous compounds across cell membranes (PMID: 11581266, 15083066). In normal liver, MRP3 was found present mainly in the bile ducts. In livers of patients with various forms of cholestasis, MRP3 levels were frequently increased in the proliferative cholangiocytes, suggesting its role in cholehepatic and enterohepatic bile circulation and in protecting liver from toxic bile salts( PMID: 11850532, 11283840). MRP3 is expressed in the liver, colon, pancreas, and, at a lower level, in the kidney (PMID:10094960). For optimal WB detection of this membrane protein, we recommend to avoid boiling the sample after lysis.

Publications

Species	Application	Title
mouse	WB	Life Sci; SMARCA4 (BRG1) activates ABCC3 transcription to promote hepatocellular carcinogenesis; Authors - Huimin Liu